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Nitrogen in dietary glutamate is exclusively utilized for the synthesis of amino acids in the rat intestine.
MedLine Citation:
PMID:  23115079     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Although previous studies have shown that virtually the entire carbon skeleton of dietary glutamate (glutamate-C) is metabolized in the gut for energy production and amino acid synthesis, little is known regarding the fate of dietary glutamate nitrogen (glutamate-N). In this study, we hypothesized that dietary glutamate-N is an effective nitrogen source for amino acid synthesis and investigated the fate of dietary glutamate-N using [(15)N]glutamate. Fischer male rats were given hourly meals containing [U-(13)C] or [(15)N]glutamate. The concentration and isotopic enrichment of several amino acids were measured after 0 to 9 h of feeding, and the net release of each amino acid into the portal vein was calculated. Most of the dietary glutamate-C was metabolized into CO(2), lactate or alanine (56%, 13% and 12% of the dietary input, respectively) in the portal drained viscera (PDV). Most of the glutamate-N was utilized for the synthesis of other amino acids such as alanine and citrulline (75% and 3% of dietary input, respectively) in the PDV, and only minor amounts were released into the portal vein in the form of ammonia and glutamate (2% and 3% of the dietary input, respectively). Substantial incorporation of (15)N into systemic amino acids such as alanine, glutamine, proline, amino acids of the urea-cycle and branched-chain amino acids was also evident. These results provide quantitative evidence that dietary glutamate-N distributed extensively to amino acids synthesized in the PDV, and consequently, to circulating amino acids.
Authors:
Hidehiro Nakamura; Yasuko Kawamata; Tomomi Kuwahara; Kunio Torii; Ryosei Sakai
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-31
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  -     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Ajinomoto Co., Inc.
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