| Nitrogen fixation system of tungsten-resistant mutants of Azotobacter vinelandii. | |
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MedLine Citation:
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PMID: 6956567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutants of Azotobacter vinelandii ATCC 12837 were isolated which could fix N2 in the presence of high tungsten concentrations. The most studied of these mutants (WD2) grew well in N-free modified Burk broth containing 10 mM W, whereas the wild type would not grow in this medium. WD2 would also grow in Burk N-free broth at about the same rate as the wild type. WD2 in broth containing W exhibited 22% of the whole cell acetylene reduction activity of the wild type in broth containing Mo and showed a lowered affinity for acetylene. Two-dimensional gel electrophoresis experiments showed that N2-fixing cells of WD2 from broth containing W or Mo did not produce significant amounts of component I of native nitrogenase protein. Electron spin resonance spectra of whole cells and cell-free extracts of WD2 from broth containing W lacked any trace of the g = 3.6 resonance associated with FeMoCo. |
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Authors:
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G D Riddle; J G Simonson; B J Hales; H D Braymer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 152 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1982 Oct |
Date Detail:
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Created Date: 1982-12-03 Completed Date: 1982-12-03 Revised Date: 2010-09-10 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 72-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylene
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metabolism Azotobacter / drug effects, genetics, metabolism* Drug Resistance, Microbial Molybdenum / pharmacology Mutation Nitrogen Fixation* Nitrogenase / analysis Oxidation-Reduction Tungsten / pharmacology* |
| Chemical | |
Reg. No./Substance:
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74-86-2/Acetylene; 7439-98-7/Molybdenum; 7440-33-7/Tungsten; EC 1.18.6.1/Nitrogenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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