| NITROGEN FIXATION BY MEMBERS OF THE TRIBE KLEBSIELLEAE. | |
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MedLine Citation:
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PMID: 14291584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mahl, M. C. (University of Wisconsin, Madison), P. W. Wilson, M. A. Fife, and W. H. Ewing. Nitrogen fixation by members of the tribe Klebsielleae. J. Bacteriol. 89:1482-1487. 1965.-Strains of species of the tribe Klebsielleae were tested for nitrogen fixation by inoculating actively growing cultures into side-arm flasks containing a medium with a growth-limiting quantity of combined nitrogen (25 mug of nitrogen per ml as ammonium sulfate). The flasks were evacuated, filled with pure N(2), sealed, and placed on a shaker at 30 C. Growth was followed by optical-density measurements; maximal growth was obtained in 9 to 10 hr. Yeast extract was then added as a source of amino acids to shorten the induction time for the nitrogen-fixing enzymes. Fixation was determined either by estimating total nitrogen with a semimicro Kjeldahl technique or by exposing 20- to 22-hr-old samples to an atmosphere of N(2) (15) and helium for 5 hr and then analyzing the digested sample for N(15) in a mass spectrometer. None of the 22 strains of the two Enterobacter (formerly Aerobacter) species fixed nitrogen; neither did any of eight strains of Serratia species that were tested. Of 31 strains of Klebsiella pneumoniae, 13 incorporated atmospheric nitrogen. Net nitrogen fixed ranged from 17 to 65 mug/ml. It is concluded that these facultative anaerobic, gram-negative, nitrogen-fixing rods should be placed in the genus Klebsiella pneumoniae. The nitrogen-fixing organism tentatively classified as Achromobacter N-4 should also be changed to K. pneumoniae strain N-4, because it has been found to be an anaerogenic strain of K. pneumoniae. |
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Authors:
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M C MAHL; P W WILSON; M A FIFE; W H EWING |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 89 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1965 Jun |
Date Detail:
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Created Date: 1965-08-01 Completed Date: 1996-12-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1482-7 Citation Subset: OM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Achromobacter* Classification* Culture Media* Enterobacter aerogenes* Klebsiella* Nitrogen Fixation* Research* Serratia* Serratia marcescens* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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