| Nitrogen excretion in developing zebrafish (Danio rerio): a role for Rh proteins and urea transporters. | |
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MedLine Citation:
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PMID: 19279128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Injection of antisense oligonucleotide morpholinos to elicit selective gene knockdown of ammonia (Rhag, Rhbg, and Rhcg1) or urea transporters (UT) was used as a tool to assess the relative importance of each transporter to nitrogen excretion in developing zebrafish (Danio rerio). Knockdown of UT caused urea excretion to decrease by approximately 90%, whereas each of the Rh protein knockdowns resulted in an approximately 50% reduction in ammonia excretion. Contrary to what has been hypothesized previously for adult fish, each of the Rh proteins appeared to have a similar effect on total ammonia excretion, and thus all are required to facilitate normal ammonia excretion in the zebrafish larva. As demonstrated in other teleosts, zebrafish embryos utilized urea to a much greater extent than adults and were effectively ureotelic until hatching. At that point, ammonia excretion rapidly increased and appeared to be triggered by a large increase in the mRNA expression of Rhag, Rhbg, and Rhcg1. Unlike the situation in the adult pufferfish (35), the various transporters are not specifically localized to the gills of the developing zebrafish, but each protein has a unique expression pattern along the skin, gills, and yolk sac. This disparate pattern of expression would appear to preclude interaction between the Rh proteins in zebrafish embryos. However, this may be a developmental feature of the delayed maturation of the gills, because as the embryos matured, expression of the transporters in and around the gills increased. |
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Authors:
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M H Braun; S L Steele; M Ekker; S F Perry |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-11 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: 296 ISSN: 1931-857X ISO Abbreviation: Am. J. Physiol. Renal Physiol. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-27 Completed Date: 2009-07-02 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: United States |
Other Details:
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Languages: eng Pagination: F994-F1005 Citation Subset: IM |
Affiliation:
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Department of Biology and Center for Advanced Research in Environmental Genetics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5. mbraun@uottawa.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ammonia
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metabolism Animals Blood Proteins / genetics, metabolism* Cation Transport Proteins / genetics, metabolism* Embryo, Nonmammalian / metabolism Female Gene Expression Regulation, Developmental / physiology Gills / embryology, physiology Kidney / embryology, physiology Male Membrane Glycoproteins / genetics, metabolism* Membrane Transport Proteins / genetics, metabolism* Nitrogen / metabolism* Oligonucleotides / pharmacology RNA, Messenger / metabolism Skin / embryology Urea / metabolism Yolk Sac / embryology, physiology Zebrafish / embryology*, metabolism Zebrafish Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Cation Transport Proteins; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Oligonucleotides; 0/RNA, Messenger; 0/Rhag protein, zebrafish; 0/Rhbg protein, zebrafish; 0/Rhcg1 protein, zebrafish; 0/Zebrafish Proteins; 0/urea transporter; 57-13-6/Urea; 7664-41-7/Ammonia; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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