Document Detail

Nitroblue tetrazolium (NBT) activity in human leucocytes after freezing.
MedLine Citation:
PMID:  643322     Owner:  NLM     Status:  MEDLINE    
Human peripheral blood leucocytes (neutrophil-rich) were collected either with heparin or acid citrate dextrose, frozen with dimethyl sulphoxide at a controlled rate, stored in liquid nitrogen at--196 degrees C and reconstituted with a solution containing dextran. After reconstitution, 20.2% of cells (in absolute numbers 1 in 5 fresh cells) showed a strongly positive nitroblue tetrazolium (NBT) reaction. The quantitative NBT test confirmed the synthesis of formazan/10(6) reconstituted neutrophilsa s15% of the fresh capacity. A slow titration reconstitution method for cells did not improve the functional capacity of thawed leucocytes as judged by the NBT test. When comparing anticoagulants, heparin increased the post-reconstitution cell yields after freezing and increased the absolute number of reconstituted cells capable of developing a positive NBT reaction.
R S Hill; M Kennedy; C Mackinder
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology     Volume:  10     ISSN:  0031-3025     ISO Abbreviation:  Pathology     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-06-12     Completed Date:  1978-06-12     Revised Date:  2006-10-31    
Medline Journal Info:
Nlm Unique ID:  0175411     Medline TA:  Pathology     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  69-75     Citation Subset:  IM    
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MeSH Terms
Blood Preservation / methods*
Dimethyl Sulfoxide
Leukocytes / metabolism*
Neutrophils / metabolism
Nitroblue Tetrazolium / metabolism*
Tetrazolium Salts / metabolism*
Reg. No./Substance:
0/Tetrazolium Salts; 298-83-9/Nitroblue Tetrazolium; 67-68-5/Dimethyl Sulfoxide

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