| Nitrite-modified extracellular matrix proteins deleteriously affect retinal pigment epithelial cell function and viability: a comparison study with nonenzymatic glycation mechanisms. | |
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MedLine Citation:
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PMID: 16109650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Extracellular matrix (ECM) plays an important role in the regulation of cell function. The aging process may involve chemical modifications to ECM proteins, which may contribute to the aging of the Bruch membrane and pathogenesis of age-related macular degeneration (AMD). The purpose of this study is to investigate nitrite modification of basement membrane-like proteins on RPE cell behavior as a model for the aging of the Bruch membrane in age-related eye diseases. As a comparison, retinal pigment epithelium (RPE) cell behavior on glycolaldehyde-modified matrices (GMM) was also studied. METHODS: Growth factor reduced Matrigel was reacted with nitrite or glycolaldehyde for 1 week or 12 hr, respectively. Calf RPE cells were plated on the modified matrices and examined in several ways. Attachment rates, proliferation rates, apoptosis, and necrosis were determined. Cell morphology and cell susceptibility to A2E-mediated damage was also monitored. RESULTS: Nitrite-modified matrices (NMMs) inhibited cell attachment by 65% and proliferation by 33.7% compared to 69.6% and 21.7%, respectively, by GMM. Proliferation inhibition was not significant when cells were plated at high density on GMM (3.47%) but significant on NMM (20.9%). NMM induced cell apoptosis and necrosis, but GMM induced cell apoptosis only. Both modifications inhibited RPE differentiation. RPE cells on both matrices were more susceptible to blue light mediated damage by A2E, but damage was greater on NMM. CONCLUSIONS: NMM has significant damaging effects on RPE cell function and viability that is similar to the damaging effects of GMM. These studies may have relevance to the RPE dysfunction observed during the progression of AMD. |
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Authors:
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Zhen Wang; David C Paik; Lucian V Del Priore; Robert L Burch; Elizabeth R Gaillard |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Current eye research Volume: 30 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-19 Completed Date: 2005-10-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 691-702 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, Illinois 60115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Basement Membrane / drug effects Cattle Cell Adhesion Cell Culture Techniques Cell Proliferation Cell Survival Collagen Drug Combinations Extracellular Matrix Proteins / metabolism* Glycosylation Laminin Necrosis Nitrosation Pigment Epithelium of Eye / cytology*, drug effects* Proteoglycans Pyridinium Compounds / toxicity Retinoids / toxicity Sodium Nitrite / toxicity* |
| Grant Support | |
ID/Acronym/Agency:
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K08 AG000863/AG/NIA NIH HHS; R01 EY12344/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/A2E compound; 0/Drug Combinations; 0/Extracellular Matrix Proteins; 0/Laminin; 0/Proteoglycans; 0/Pyridinium Compounds; 0/Retinoids; 119978-18-6/matrigel; 7632-00-0/Sodium Nitrite; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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