| Nitrite in exhaled breath condensate as a marker of nitrossative stress in the airways of patients with asthma, COPD, and idiopathic pulmonary fibrosis. | |
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MedLine Citation:
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PMID: 20872566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Nitrite and nitrate are exhaled in droplets of an aerosol during breathing and can be assayed in the exhaled breath condensate (EBC) as markers of nitrossative stress in the airways of patients with asthma, COPD, and idiopathic pulmonary fibrosis (IPF). SUBJECTS AND METHODS: Using HPLC with fluorescence detection, nitrite and nitrate were assayed in EBC of 14 atopic patients with mild-to-moderate stable asthma, 18 atopic asthmatics with exacerbation, 14 COPD patients without exacerbation, 18 patients with exacerbated COPD, 13 patients with active IPF, and in 29 healthy subjects. RESULTS: The geometric mean [exp(mean±SD)] EBC concentrations of nitrite (micromol/l) in patients with asthma [5.1(2.1-12.3)], exacerbation of asthma [5.1(2.8-9.6)], exacerbation of COPD [5.3(3.2-8.7)], and with IPF [5.5(2.9-10.2)] were higher (P<0.05) compared with those of healthy subjects [2.9(1.6-5.3)] and patients with stable COPD [3.0(1.3-6.7)]. Nitrite concentration increased with decreased lung function of patients with asthma (r(s)=-0.31, P<0.02). Presumably owing to the contamination of the EBC sample with nitrate during collection, nitrate levels were highly variable among healthy subjects and higher compared with all groups of patients. CONCLUSION: EBC nitrite is a suitable marker of nitrossative stress in adult patients with lung diseases but cannot differentiate controlled and exacerbated asthma. Further improvements to the methods of EBC collection and sample handling are warranted. |
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Authors:
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Vladimír Rihák; Petr Zatloukal; Jirina Chládková; Alena Zimulová; Zuzana Havlínová; Jaroslav Chládek |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical laboratory analysis Volume: 24 ISSN: 1098-2825 ISO Abbreviation: J. Clin. Lab. Anal. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2011-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8801384 Medline TA: J Clin Lab Anal Country: United States |
Other Details:
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Languages: eng Pagination: 317-22 Citation Subset: IM |
Copyright Information:
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J. Clin. Lab. Anal. 24:317-322, 2010. © 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Respiratory Diseases, Regional Thomas Bata Hospital, Havlíckovo nábrezí, Zlín, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Asthma / metabolism* Biological Markers / metabolism* Breath Tests Case-Control Studies Exhalation Female Humans Idiopathic Pulmonary Fibrosis / metabolism* Male Middle Aged Nitrites / metabolism* Pulmonary Disease, Chronic Obstructive / metabolism* Respiratory System Stress, Physiological* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Nitrites |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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