| Nitric oxide synthase gene knockout mice do not become hypertensive during pregnancy. | |
| | |
MedLine Citation:
|
PMID: 11717657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: The purpose of this study was to test whether omitting the vasodilator nitric oxide that is derived from any 1 of the 3 isoforms of nitric oxide synthase results in hypertension during pregnancy. STUDY DESIGN: We measured systolic blood pressure before, during, and after pregnancy using an automated tail cuff method in 3 mutant (gene knockout) mouse strains in which the gene for neuronal nitric oxide, inducible nitric oxide, or endothelial nitric oxide was disrupted by gene targeting. RESULTS: In neuronal nitric oxide gene knockout mice (n = 10), blood pressure was 100 +/- 3 mm Hg, not significantly different from 101 +/- 3 mm Hg in matched wild-type control mice (n = 10). Pregnancy did not change blood pressure or heart rate in either group. In inducible nitric oxide gene knockout mice (n = 9), blood pressure was 110 +/- 3 mm Hg, the same as in the wild-type control mice (110 +/- 2 mm Hg; n = 14). Blood pressure was unaffected by pregnancy in either group of mice. However, heart rate was significantly less in knockout mice (647 +/- 11 beats/min vs 666 +/- 9 beats/min; P <.005); this difference persisted through pregnancy. In endothelial nitric oxide gene knockout mice (n = 8), blood pressure was higher before pregnancy (114 +/- 4 mm Hg vs 103 +/- 4 mm Hg; P <.05) than in wild-type control mice (n = 9), but this difference disappeared during pregnancy, returning only after delivery. Heart rates were not different before pregnancy and were unaffected by pregnancy. CONCLUSION: There was no apparent increase in systolic blood pressure in any of the 3 nitric oxide synthase gene knockout strains during pregnancy compared to the wild-type control mice. This suggests that, at least in the mouse, genetic deficiency of any 1 isoform of nitric oxide synthase does not result in pregnancy-induced hypertension. |
| | |
Authors:
|
E G Shesely; C Gilbert; G Granderson; C D Carretero; O A Carretero; W H Beierwaltes |
Related Documents
:
|
10981117 - Hypertension in pregnancy: new recommendations for management. 15221327 - Bilateral pheochromocytoma during pregnancy. 16959687 - C-reactive protein in hypertensive disorders in pregnancy. 8536057 - Atrial natriuretic peptide (anp) in preeclampsia-like syndrome in a rat model. 577127 - The effect of prostaglandin f2alpha on endocrine parameters in early pregnancy. 5440587 - Haematemesis and melaena, with special reference to factors influencing the outcome. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: American journal of obstetrics and gynecology Volume: 185 ISSN: 0002-9378 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2001 Nov |
Date Detail:
|
Created Date: 2001-11-21 Completed Date: 2001-12-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
|
Languages: eng Pagination: 1198-203 Citation Subset: AIM; IM |
Affiliation:
|
Hypertension and Vascular Research Division, Henry Ford Hospital, Case Western Reserve University Detroit Medical Campus, Mich 48202-2689, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Pressure / physiology* Female Mice Mice, Knockout / genetics Nitric Oxide Synthase / deficiency*, genetics, physiology Nitric Oxide Synthase Type I Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Pregnancy Pregnancy, Animal / physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
HL28982/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos1 protein, mouse; EC 1.14.13.39/Nos2 protein, mouse; EC 1.14.13.39/Nos3 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protein expression of phospholipase C in pregnant and nonpregnant rat uterine tissue.
Next Document: Intrapartum management of nonvertex second-born twins: a critical analysis.