| Nitric oxide synthase activity in Fasciola hepatica: a radiometric study. | |
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MedLine Citation:
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PMID: 12866797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The activity of neuronal nitric oxide synthase (nNOS) in homogenates of adult Fasciola hepatica was measured by the direct radiometric assay of the production of L-[3H]citrulline. This is the first radiometric study of the activity of nNOS in a fluke. The effect of arginase was tested. In the presence of L-valine, which is an inhibitor of arginase, the formation of L-[3H]citrulline decreased from 12% to 38%, depending on the time of incubation. This means that the arginase activity in the worm is high, and has to be taken into consideration when measuring the activity of nNOS. When co-factors, such as H4B, and NADPH, were omitted the formation of L-[3H]citrulline decreased significantly (29%). The effects of several nNOS inhibitors were tested. N(omega)-nitro-L-arginine (L-NAME), aminoguanidine and S-methyl-L-thiocitrulline added at a concentration of 1 mM inhibited the L-[3H]citrulline formation by 28%, 15% and 14%, respectively. Chelation of Ca2+ with 1 mM EGTA resulted in a 40% decrease in the formation of L-[3H]citrulline. These results indicate the presence of nNOS activity in homogenates of F. hepatica. |
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Authors:
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N B Terenina; M V Onufriev; N V Gulyaeva; Y V Moiseeva; M K S Gustafsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Parasitology Volume: 126 ISSN: 0031-1820 ISO Abbreviation: Parasitology Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-07-17 Completed Date: 2003-11-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0401121 Medline TA: Parasitology Country: England |
Other Details:
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Languages: eng Pagination: 585-90 Citation Subset: IM |
Affiliation:
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Institute of Parasitology, Russian Academy of Sciences, Lenin Avenue 33, 117071 Moscow, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginase / antagonists & inhibitors, metabolism Cattle Citrulline / analogs & derivatives*, biosynthesis, pharmacology Enzyme Inhibitors / pharmacology Fasciola hepatica / enzymology* Guanidines / pharmacology NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / analysis*, antagonists & inhibitors Radiometry Thiourea / analogs & derivatives*, pharmacology Valine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Guanidines; 156719-41-4/S-methylthiocitrulline; 372-75-8/Citrulline; 50903-99-6/NG-Nitroarginine Methyl Ester; 62-56-6/Thiourea; 7004-03-7/Valine; 79-17-4/pimagedine; EC 1.14.13.39/Nitric Oxide Synthase; EC 3.5.3.1/Arginase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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