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Nitric oxide suppresses L-type calcium currents in basilar artery smooth muscle cells in rabbits.
MedLine Citation:
PMID:  23540411     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVES: Nitric oxide (NO) is well known to be a vasodilator, and NO donor compounds are currently used for treating vasospasm following subarachnoid hemorrhage. However, the action mechanism of cerebral vascular relaxation is not yet clear. L-type calcium channels have been determined to play an essential role in smooth muscle contraction. To investigate the role of L-type calcium channels in NO-induced relaxation of basilar smooth muscle cells, we examined the effect of the NO donor, sodium nitroprusside (SNP) on calcium (Ca(2+)) currents using smooth muscle cells isolated from a rabbit basilar artery.
METHOD: The smooth muscle cells were isolated from rabbit basilar artery by enzyme treatment. To identify L-type Ca(2+) currents, we used cesium chloride, a potassium channel blocker and Bay K8644, an activator of L-type Ca(2+) channel.
RESULTS: The L-type calcium currents (91±13·0 pA; n = 11) were significantly reduced by SNP (32±5 pA; n = 11; P<0·05). 1H-[1,2,4] Oxadiazolo [4,3-a] quinoxalin-1-one, a 3',5'-cyclic guanosine monophosphate inhibitor, blocked the effect of SNP on L-type Ca(2+) currents, and similar results were obtained after the application of 7-nitroindazole, a specific NO synthase inhibitor. Furthermore, inward currents were enhanced by Bay K8644 (170±22 pA; n = 5) and were suppressed by SNP (54±13 pA; n = 5; P<0·05).
DISCUSSION: These results demonstrate that NO suppresses the L-type Ca(2+) currents in rabbit basilar smooth muscle cells, and suggest that L-type Ca(2+) channels may play a pivotal role in NO-induced vascular relaxation.
Naveen Sharma; Janardhan Prasad Bhattarai; Pyoung Han Hwang; Seong Kyu Han
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurological research     Volume:  35     ISSN:  1743-1328     ISO Abbreviation:  Neurol. Res.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905298     Medline TA:  Neurol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  424-8     Citation Subset:  IM    
Chonbuk National University, Jeonju, Korea.
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