| Nitric oxide and superoxide anion production during endothelial cell proliferation. | |
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MedLine Citation:
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PMID: 8944635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously reported that nitric oxide (NO) synthase activity, protein, and mRNA are increased in proliferating compared with postconfluent bovine aortic endothelial cells (BAEC). Because superoxide anion inactivates NO, in the present study, we have assessed the effect of proliferation on superoxide anion production by use of cytochrome c reduction. The superoxide anion production in proliferating cells was increased about threefold compared with postconfluent cells in both basal and calcium ionophore-stimulated conditions and exceeded the amount of released nitrite and nitrate (NOx) in all cases. A-23187 (1 microM) stimulated the superoxide anion production about twofold at all stages of confluence. Because superoxide anion can inactivate NO, we then assessed the effect of proliferation on NO bioactivity released in the conditioned medium, by use of RFL-6 cells (reporter cells very rich in guanylate cyclase, which on activation by NO generates guanosine 3',5'-cyclic monophosphate, second messenger of NO). In the absence of added superoxide dismutase (SOD) in the conditioned medium, the guanylate cyclase-stimulating activities evoked by A-23187 from proliferating and growth-arrested cells were similar, despite a greater NOx release in the former. When SOD (100 U/ml) was added in the conditioned medium, the guanylate cyclase-stimulating activity evoked by 1 microM A-23187 was increased approximately 10-fold and closely paralleled NOx release (i.e., was greater in supernatant of proliferating cells than in that of growth-arrested cells). Thus BAEC release more superoxide anion extracellularly than NO at all stages of confluence. Endothelium-derived superoxide anion is a major determinant of the breakdown of NO. |
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Authors:
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J F Arnal; I Tack; J P Besombes; B Pipy; A Nègre-Salvayre |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-01-09 Completed Date: 1997-01-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C1521-6 Citation Subset: IM |
Affiliation:
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Laboratoire de Physiologie, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta Biological Assay Calcimycin / pharmacology Cattle Cell Division / drug effects Cell Line Cells, Cultured Cytochrome c Group / metabolism Endothelium, Vascular / cytology*, drug effects, metabolism* Guanylate Cyclase / metabolism Kinetics Nitric Oxide / analysis, biosynthesis* Nitric Oxide Synthase / metabolism* Oxidation-Reduction Superoxides / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cytochrome c Group; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 52665-69-7/Calcimycin; EC 1.14.13.39/Nitric Oxide Synthase; EC 4.6.1.2/Guanylate Cyclase |
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