Document Detail


Nitric oxide stimulation of cGMP accumulation in myometrial cells from pregnant women is antagonized by oxytocin.
MedLine Citation:
PMID:  19544684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of cGMP in the myometrium of pregnant women is not completely known. We have previously shown in guinea pig, monkey and man that NO-induced relaxation of oxytocin-induced contractions is independent of cGMP accumulation. To approach an understanding of the role of cGMP in myometrium, we have developed smooth muscle cell cultures from pregnant women undergoing caesarian section at term. Cells, grown in standard media containing progesterone, express smooth muscle cell markers and are used within five doublings. Cells stimulated with NO donors increase their cGMP levels nearly 100 fold (basal = approximately 9 pmol/mg protein). In the presence of oxytocin (OT; 1 microM), cGMP accumulation in the presence of NO (100 microM) is significantly blunted (25 fold). Cyclic GMP-degradation is inhibited by the presence of the phosphodiesterase inhibitor zaprinast; suggesting that the ability of OT to attenuate cGMP accumulation is unlikely to be due to degradation. We propose that the elevation of intracellular calcium following the addition of OT suppress the activity of a calcium-sensitive guanylyl cyclase. The diminution of cGMP synthetic potential in myometrial cells from pregnant women is consistent with the absence of a role for cGMP in the NO-induced relaxation of uterine muscle.
Authors:
Iain L O Buxton
Related Documents :
18181174 - Vascular smooth muscle cell polyploidy: an adaptive or maladaptive response?
10506584 - Constitutive expressions of type i nos in human airway smooth muscle cells: evidence fo...
4515934 - Evidence for a monoclonal origin of human atherosclerotic plaques.
7750644 - Testosterone treatment results in quiescent satellite cells being activated and recruit...
10490284 - Redox modulation of cell surface protein thiols in u937 lymphoma cells: the role of gam...
8844014 - Tar- and tat-independent replication of human immunodeficiency virus type 1 in human he...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Proceedings of the Western Pharmacology Society     Volume:  51     ISSN:  0083-8969     ISO Abbreviation:  Proc. West. Pharmacol. Soc.     Publication Date:  2008  
Date Detail:
Created Date:  2009-06-23     Completed Date:  2009-08-10     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  7505899     Medline TA:  Proc West Pharmacol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-82     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557, USA. ibuxton@medicine.nevada.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Cyclic GMP / agonists,  antagonists & inhibitors,  metabolism*
Female
Humans
Myocytes, Smooth Muscle / drug effects*,  metabolism*
Myometrium / drug effects*,  metabolism*
Nitric Oxide / pharmacology*
Oxytocin / pharmacology*
Phosphodiesterase Inhibitors / pharmacology
Pregnancy
Purinones / pharmacology
Grant Support
ID/Acronym/Agency:
HD053028/HD/NICHD NIH HHS; R01 HD053028-01A1/HD/NICHD NIH HHS; R01 HD053028-02/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 0/Purinones; 10102-43-9/Nitric Oxide; 37762-06-4/zaprinast; 50-56-6/Oxytocin; 7665-99-8/Cyclic GMP
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Persistent pulmonary hypertension in the newborn: therapeutic effect of sildenafil.
Next Document:  Breast cancer in women under 40.