Document Detail

Nitric oxide stimulation of cGMP accumulation in myometrial cells from pregnant women is antagonized by oxytocin.
MedLine Citation:
PMID:  19544684     Owner:  NLM     Status:  MEDLINE    
The role of cGMP in the myometrium of pregnant women is not completely known. We have previously shown in guinea pig, monkey and man that NO-induced relaxation of oxytocin-induced contractions is independent of cGMP accumulation. To approach an understanding of the role of cGMP in myometrium, we have developed smooth muscle cell cultures from pregnant women undergoing caesarian section at term. Cells, grown in standard media containing progesterone, express smooth muscle cell markers and are used within five doublings. Cells stimulated with NO donors increase their cGMP levels nearly 100 fold (basal = approximately 9 pmol/mg protein). In the presence of oxytocin (OT; 1 microM), cGMP accumulation in the presence of NO (100 microM) is significantly blunted (25 fold). Cyclic GMP-degradation is inhibited by the presence of the phosphodiesterase inhibitor zaprinast; suggesting that the ability of OT to attenuate cGMP accumulation is unlikely to be due to degradation. We propose that the elevation of intracellular calcium following the addition of OT suppress the activity of a calcium-sensitive guanylyl cyclase. The diminution of cGMP synthetic potential in myometrial cells from pregnant women is consistent with the absence of a role for cGMP in the NO-induced relaxation of uterine muscle.
Iain L O Buxton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Proceedings of the Western Pharmacology Society     Volume:  51     ISSN:  0083-8969     ISO Abbreviation:  Proc. West. Pharmacol. Soc.     Publication Date:  2008  
Date Detail:
Created Date:  2009-06-23     Completed Date:  2009-08-10     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  7505899     Medline TA:  Proc West Pharmacol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-82     Citation Subset:  IM    
Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557, USA.
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MeSH Terms
Cell Culture Techniques
Cyclic GMP / agonists,  antagonists & inhibitors,  metabolism*
Myocytes, Smooth Muscle / drug effects*,  metabolism*
Myometrium / drug effects*,  metabolism*
Nitric Oxide / pharmacology*
Oxytocin / pharmacology*
Phosphodiesterase Inhibitors / pharmacology
Purinones / pharmacology
Grant Support
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 0/Purinones; 10102-43-9/Nitric Oxide; 37762-06-4/zaprinast; 50-56-6/Oxytocin; 7665-99-8/Cyclic GMP

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