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Nitric oxide signaling is involved in diarylheptanoid-induced increases in femoral arterial blood flow in ovariectomised rats.
MedLine Citation:
PMID:  23331131     Owner:  NLM     Status:  Publisher    
The mechanisms by which the hexane extract of C. comosa increases femoral blood flow (FBF) in ovariectomised rats are not known. This study, therefore, investigated the acute effects and modes of action of a diarylhaptanoid (D3: (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol), a phyto-oestrogen isolated from C. comosa, on FBF in ovariectomised rats. On day 7 after ovariectomy, three groups of rats were intra-arterially injected with D3 (100, 200, 400, and 800 μg/kg body weight (BW)), 17-β-oestradiol (E2) (1, 2, 4, and 8 μg/kg BW) or vehicle. Mean arterial blood pressure (mABP) and FBF were recorded using a pressure transducer and a Laser Doppler Flow meter, respectively. D3 and E2 dose dependently increased FBF without any alterations in mABP and heart rate. The EC(50) at 120 min for D3 and E2 were 195.8 μg/kg BW and 1.8 μg/kg BW, respectively. Both D3 and E2 also dose-dependently decreased femoral vascular resistance (FVR). The EC(50) of D3 was about 100-fold greater than that of E2. The effects of D3 and E2 on FBF and FVR were diminished after intravenous injection of N(G) -nitro-L-arginine methyl ester (L-NAME) (10 mg/kg BW). Pretreatment with L-NAME (10 mg/kg BW), 1 H-[1,2,4] oxadiazolo-[4,3-a] quinoxalin-1-one (ODQ) (900 μg/kg BW) or ICI 128,780 (900 μg/kg BW) for 30 minutes prevented the effects of D3 and E2 on FBF and FVR. The results suggest that a phyto-oestrogen, D3, increases FBF in ovariectomised rats via oestrogen receptor and NO-guanylyl cyclase signaling, which, in turn, relaxes the femoral vascular resistance. © 2013 The Authors Clinical and Experimental Pharmacology and Physiology © 2013 Wiley Publishing Asia Pty Ltd.
Ganyapong Chaturapanich; Rungsima Yamthed; Pawinee Piyachaturawat; Arthit Chairoungdua; Wisuda Suvitayavat; Boontium Kongsaktrakoon; Apichart Suksamrarn; Chumpol Pholpramool
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  -     ISSN:  1440-1681     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors Clinical and Experimental Pharmacology and Physiology © 2013 Wiley Publishing Asia Pty Ltd.
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
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