Document Detail


Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation.
MedLine Citation:
PMID:  23281369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cysteine S-nitrosylation is a posttranslational modification by which nitric oxide regulates protein function and signaling. Studies of individual proteins have elucidated specific functional roles for S-nitrosylation, but knowledge of the extent of endogenous S-nitrosylation, the sites that are nitrosylated, and the regulatory consequences of S-nitrosylation remains limited. We used mass spectrometry-based methodologies to identify 1011 S-nitrosocysteine residues in 647 proteins in various mouse tissues. We uncovered selective S-nitrosylation of enzymes participating in glycolysis, gluconeogenesis, tricarboxylic acid cycle, and oxidative phosphorylation, indicating that this posttranslational modification may regulate metabolism and mitochondrial bioenergetics. S-nitrosylation of the liver enzyme VLCAD [very long chain acyl-coenzyme A (CoA) dehydrogenase] at Cys(238), which was absent in mice lacking endothelial nitric oxide synthase, improved its catalytic efficiency. These data implicate protein S-nitrosylation in the regulation of β-oxidation of fatty acids in mitochondria.
Authors:
Paschalis-Thomas Doulias; Margarita Tenopoulou; Jennifer L Greene; Karthik Raju; Harry Ischiropoulos
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-01
Journal Detail:
Title:  Science signaling     Volume:  6     ISSN:  1937-9145     ISO Abbreviation:  Sci Signal     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-06-05     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101465400     Medline TA:  Sci Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  rs1     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acyl-CoA Dehydrogenase, Long-Chain / metabolism
Analysis of Variance
Animals
Cysteine / analogs & derivatives*,  metabolism
DNA Primers / genetics
Energy Metabolism / physiology*
Fatty Acids / metabolism*
Liver / anatomy & histology,  metabolism
Mass Spectrometry
Mice
Mitochondria / metabolism*
Mutagenesis, Site-Directed
Nitric Oxide / metabolism*
Oxidation-Reduction
Protein Processing, Post-Translational / genetics,  physiology*
Proteomics
S-Nitrosothiols / metabolism*
Signal Transduction / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
AG13966/AG/NIA NIH HHS; ES013508/ES/NIEHS NIH HHS; HL054926/HL/NHLBI NIH HHS; R01 AG013966/AG/NIA NIH HHS; R01 HL054926/HL/NHLBI NIH HHS; T32AG000255/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Fatty Acids; 0/S-Nitrosothiols; 31C4KY9ESH/Nitric Oxide; 51209-75-7/S-nitrosocysteine; EC 1.3.8.8/Acyl-CoA Dehydrogenase, Long-Chain; K848JZ4886/Cysteine
Comments/Corrections

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