| Nitric oxide regulates mitochondrial Fatty Acid metabolism through reversible protein s-nitrosylation. | |
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MedLine Citation:
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PMID: 23281369 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Cysteine S-nitrosylation is a posttranslational modification by which nitric oxide regulates protein function and signaling. Studies of individual proteins have elucidated specific functional roles for S-nitrosylation, but knowledge of the extent of endogenous S-nitrosylation, the sites that are nitrosylated, and the regulatory consequences of S-nitrosylation remains limited. We used mass spectrometry-based methodologies to identify 1011 S-nitrosocysteine residues in 647 proteins in various mouse tissues. We uncovered selective S-nitrosylation of enzymes participating in glycolysis, gluconeogenesis, tricarboxylic acid cycle, and oxidative phosphorylation, indicating that this posttranslational modification may regulate metabolism and mitochondrial bioenergetics. S-nitrosylation of the liver enzyme VLCAD [very long chain acyl-coenzyme A (CoA) dehydrogenase] at Cys(238), which was absent in mice lacking endothelial nitric oxide synthase, improved its catalytic efficiency. These data implicate protein S-nitrosylation in the regulation of β-oxidation of fatty acids in mitochondria. |
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Authors:
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Paschalis-Thomas Doulias; Margarita Tenopoulou; Jennifer L Greene; Karthik Raju; Harry Ischiropoulos |
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Publication Detail:
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Type: Journal Article Date: 2013-01-01 |
Journal Detail:
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Title: Science signaling Volume: 6 ISSN: 1937-9145 ISO Abbreviation: Sci Signal Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465400 Medline TA: Sci Signal Country: United States |
Other Details:
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Languages: eng Pagination: rs1 Citation Subset: IM |
Affiliation:
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Children's Hospital of Philadelphia Research Institute and Departments of Pediatrics and Pharmacology, Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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