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Nitric oxide regulates mitochondrial Fatty Acid metabolism through reversible protein s-nitrosylation.
MedLine Citation:
PMID:  23281369     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cysteine S-nitrosylation is a posttranslational modification by which nitric oxide regulates protein function and signaling. Studies of individual proteins have elucidated specific functional roles for S-nitrosylation, but knowledge of the extent of endogenous S-nitrosylation, the sites that are nitrosylated, and the regulatory consequences of S-nitrosylation remains limited. We used mass spectrometry-based methodologies to identify 1011 S-nitrosocysteine residues in 647 proteins in various mouse tissues. We uncovered selective S-nitrosylation of enzymes participating in glycolysis, gluconeogenesis, tricarboxylic acid cycle, and oxidative phosphorylation, indicating that this posttranslational modification may regulate metabolism and mitochondrial bioenergetics. S-nitrosylation of the liver enzyme VLCAD [very long chain acyl-coenzyme A (CoA) dehydrogenase] at Cys(238), which was absent in mice lacking endothelial nitric oxide synthase, improved its catalytic efficiency. These data implicate protein S-nitrosylation in the regulation of β-oxidation of fatty acids in mitochondria.
Authors:
Paschalis-Thomas Doulias; Margarita Tenopoulou; Jennifer L Greene; Karthik Raju; Harry Ischiropoulos
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Publication Detail:
Type:  Journal Article     Date:  2013-01-01
Journal Detail:
Title:  Science signaling     Volume:  6     ISSN:  1937-9145     ISO Abbreviation:  Sci Signal     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465400     Medline TA:  Sci Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  rs1     Citation Subset:  IM    
Affiliation:
Children's Hospital of Philadelphia Research Institute and Departments of Pediatrics and Pharmacology, Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
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