Document Detail

Nitric oxide promotes in vitro interstitial cell heart valve repair.
MedLine Citation:
PMID:  15710286     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The cell and molecular biology of heart valve wound repair is not well understood. Valve interstitial cells (IC) are thought to play an important role in valvular wound repair. Because nitric oxide (NO) has been implicated in wound repair, we tested the hypothesis that NO promotes valvular wound repair by examining the presence of the inducible form of nitric oxide synthase (iNOS) in wounded IC monolayers, in vitro. METHODS: Linear denuding wounds were made in confluent monolayers of porcine mitral valve IC plated on glass coverslips. Cultures were fixed at various times (0 to 48 h postwounding), and iNOS was localized in the cells by immunofluorescence microscopy. Cultures were also incubated with iNOS inhibitors L-N(G)-nitroarginine methyl ester (L-NAME) and N-(3-(Aminomethyl)benzyl)acetamidine (1400W), and the extent of wound closure with and without inhibitor was measured at 24, 48 and 72 h postwounding. RESULTS: From 6 to 24 h postwounding, iNOS localization was increased at the wound edge. At 48 h, iNOS was localized beyond the wound edge, into the monolayer, where the intensity of the signal gradually diminished until it was virtually imperceptible. At 24 and 48 h, the inhibition of iNOS with both L-NAME and 1400W resulted in a significant delay in wound closure. CONCLUSION: NO promotes valve wound repair through an effect on IC migration.
Adam Durbin; Nur-Ain Nadir; Alan Rosenthal; Avrum I Gotlieb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology     Volume:  14     ISSN:  1054-8807     ISO Abbreviation:  Cardiovasc. Pathol.     Publication Date:    2005 Jan-Feb
Date Detail:
Created Date:  2005-02-15     Completed Date:  2005-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9212060     Medline TA:  Cardiovasc Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12-8     Citation Subset:  IM    
Toronto General Research Institute, Toronto, ON, Canada.
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MeSH Terms
Amidines / pharmacology
Benzylamines / pharmacology
Cell Movement / drug effects
Cell Proliferation / drug effects
Cells, Cultured
Enzyme Inhibitors / pharmacology
Fluorescent Antibody Technique, Indirect
Mitral Valve / cytology*,  enzymology,  injuries
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / biosynthesis*
Nitric Oxide Synthase / antagonists & inhibitors,  metabolism
Nitric Oxide Synthase Type II
Wound Healing / drug effects*,  physiology
Reg. No./Substance:
0/Amidines; 0/Benzylamines; 0/Enzyme Inhibitors; 0/N-(3-(aminomethyl)benzyl)acetamidine; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; EC Oxide Synthase; EC Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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