Document Detail

Nitric oxide production is higher in rat cardiac microvessel endothelial cells than ventricular cardiomyocytes in baseline and hypoxic conditions: a comparative study.
MedLine Citation:
PMID:  16354719     Owner:  NLM     Status:  MEDLINE    
AIMS: To assess and compare baseline and hypoxia-induced NO and ONOO- production in cardiomyocytes and CMEC.
METHODS: Rat cardiomyocytes were isolated, and cultured rat CMEC were purchased commercially. Hypoxia (+/- NOS inhibitors) was induced by mineral oil layering or hypoxic culture. NO and ONOO- were detected by FACS analysis of DAF-2/DA and DHR123, respectively. Total eNOS was determined by Western blot analysis.
RESULTS: 1) Baseline NO production in CMEC was sevenfold (cultured cells) and 26-fold (isolated cells) higher than in cardiomyocytes, 2) eNOS expression was 22-fold higher in CMEC, 3) hypoxia increased NO production in both cell types, albeit to a larger extent in CMEC, 4) in hypoxic cardiomyocytes, nonselective NOS and iNOS-specific inhibition attenuated NO production, whereas in CMEC, iNOS-specific inhibition was ineffective, and 5) baseline ONOO- production was 2.2 times greater in CMEC than in cardiomyocytes.
CONCLUSION: Using a novel approach, this study demonstrated that CMEC produce more baseline NO than cardiomyocytes, and that hypoxia activates NOS to increase NO production in both cell types. Baseline eNOS content was higher in CMEC than in cardiomyocytes, suggesting that differences in baseline NO production were eNOS-associated.
Hans Strijdom; Sean Jacobs; Suzél Hattingh; Carine Page; Amanda Lochner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-14
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  20     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-01     Completed Date:  2006-03-15     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  314-6     Citation Subset:  IM    
Department of Medical Physiology and Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, Republic of South Africa.
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MeSH Terms
Anoxia / metabolism*
Coronary Vessels / cytology
Endothelial Cells / metabolism*
Myocytes, Cardiac / metabolism*
Nitric Oxide / biosynthesis,  metabolism*
Nitric Oxide Synthase Type III / metabolism
Peroxynitrous Acid / metabolism
Rats, Wistar
Reg. No./Substance:
10102-43-9/Nitric Oxide; 14691-52-2/Peroxynitrous Acid; EC Oxide Synthase Type III

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