Document Detail

Nitric oxide produced by ultraviolet-irradiated keratinocytes stimulates melanogenesis.
MedLine Citation:
PMID:  9045865     Owner:  NLM     Status:  MEDLINE    
Ultraviolet (UV) radiation is the main physiological stimulus for human skin pigmentation. Within the epidermal-melanin unit, melanocytes synthesize and transfer melanin to the surrounding keratinocytes. Keratinocytes produce paracrine factors that affect melanocyte proliferation, dendricity, and melanin synthesis. In this report, we show that normal human keratinocytes secrete nitric oxide (NO) in response to UVA and UVB radiation, and we demonstrate that the constitutive isoform of keratinocyte NO synthase is involved in this process. Next, we investigate the melanogenic effect of NO produced by keratinocytes in response to UV radiation using melanocyte and keratinocyte cocultures. Conditioned media from UV-exposed keratinocytes stimulate tyrosinase activity of melanocytes. This effect is reversed by NO scavengers, suggesting an important role for NO in UV-induced melanogenesis. Moreover, melanocytes respond to NO-donors by decreased growth, enhanced dendricity, and melanogenesis. The rise in melanogenesis induced by NO-generating compounds is associated with an increased amount of both tyrosinase and tyrosinase-related protein 1. These observations suggest that NO plays an important role in the paracrine mediation of UV-induced melanogenesis.
C Roméro-Graillet; E Aberdam; M Clément; J P Ortonne; R Ballotti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  99     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  635-42     Citation Subset:  AIM; IM    
Institut National de la Santé et de la Recherche Médicale U385, Faculté de Médecine, Nice, France.
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MeSH Terms
Cell Division / drug effects
Cell Size / drug effects
Cells, Cultured
Culture Media, Conditioned
Keratinocytes / enzymology,  metabolism*,  radiation effects*
Melanins / biosynthesis*,  metabolism
Melanocytes / cytology,  drug effects
Nitric Oxide / biosynthesis*,  physiology,  radiation effects
Nitric Oxide Synthase / chemistry
Ultraviolet Rays
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Melanins; 10102-43-9/Nitric Oxide; EC Oxide Synthase

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