| Nitric oxide and peroxynitrite released by ultraviolet B-irradiated human endothelial cells are possibly involved in skin erythema and inflammation. | |
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MedLine Citation:
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PMID: 8960707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we attempted to demonstrate whether endothelial cell nitric oxide synthase (eNOS) and xanthine oxidase (XO) could be activated to release nitric oxide (NO) and peroxynitrite (ONOO-) following exposure to ultraviolet B (UVB) radiation and to define whether this light-induced response could be involved in the pathogenesis of sunburn erythema and inflammation. Treatment of human endothelial cells with UVB (290-320 nm) radiation (up to 100 mJ/cm2) resulted in an increase of both NO and ONOO- release that was inhibited by NG-monomethyl-L-arginine (L-NMMA). Treatment of cell cytosol with various doses of UVB radiation (up to 20 mJ/cm2) resulted in a threefold increase of XO activity that was inhibited (approximately 90% by oxypurinol. In reconstitution experiments, when purified eNOS was added to purified XO, an almost fourfold increase in ONOO- production at 20 mj/cm2 UVB radiation was observed. UVB radiation (100 mg/cm2) decreased cell membrane fluidity, indicating changes in the physicochemical characteristics of the membranes. In in vivo experiments, when human volunteers were subjected to UVB light, a protection factor (PF) of 3.90 +/- 0.85 was calculated when an emulsified cream formulation containing nitro-L-arginine (L-NA; 2%) and L-NMMA (2%) was applied to their skin. The present studies indicate that UVB radiation acts as a potent stimulator of eNOS and XO in human endothelial cells. The cytotoxic effects of NO and ONOO- may be the main factors in the integrated response of the skin leading to vasodilatation, the first key event of erythema production and the inflammation process. |
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Authors:
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G Deliconstantinos; V Villiotou; J C Stavrides |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental physiology Volume: 81 ISSN: 0958-0670 ISO Abbreviation: Exp. Physiol. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-02-24 Completed Date: 1997-02-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9002940 Medline TA: Exp Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1021-33 Citation Subset: IM |
Affiliation:
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Department of Experimental Physiology, University of Athens Medical School, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Dermatitis / etiology, metabolism Endothelium, Vascular / metabolism, radiation effects* Enzyme Activation Erythema / etiology, metabolism Humans Nitrates / metabolism* Nitric Oxide / biosynthesis* Nitric Oxide Synthase / metabolism Sunburn / complications Ultraviolet Rays* Umbilical Veins / metabolism, radiation effects Xanthine Oxidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Nitrates; 10102-43-9/Nitric Oxide; 26404-66-0/peroxynitric acid; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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