Document Detail


Nitric oxide mediates the fungal elicitor-induced puerarin biosynthesis in Pueraria thomsonii Benth. suspension cells through a salicylic acid (SA)-dependent and a jasmonic acid (JA)-dependent signal pathway.
MedLine Citation:
PMID:  16989284     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitric oxide (NO) has emerged as a key signaling molecule in plant secondary metabolite biosynthesis recently. In order to investigate the molecular basis of NO signaling in elicitor-induced secondary metabolite biosynthesis of plant cells, we determined the contents of NO, salicylic acid (SA), jasmonic acid (JA), and puerarin in Pueraria thomsonii Benth. suspension cells treated with the elicitors prepared from cell walls of Penicillium citrinum. The results showed that the fungal elicitor induced NO burst, SA accumulation and puerarin production of P. thomsonii Benth. cells. The elicitor-induced SA accumulation and puerarin production was suppressed by nitric oxide specific scavenger cPITO, indicating that NO was essential for elicitor-induced SA and puerarin biosynthesis in P. thomsonii Benth. cells. In transgenic NahG P. thomsonii Benth. cells, the fungal elicitor also induced puerarin biosynthesis, NO burst, and JA accumulation, though the SA biosynthesis was impaired. The elicitor-induced JA accumulation in transgenic cells was blocked by cPITO, which suggested that JA acted downstream of NO and its biosynthesis was controlled by NO. External application of NO via its donor sodium nitroprusside (SNP) enhanced puerarin biosynthesis in transgenic NahG P. thomsonii Benth. cells, and the NO-triggered puerarin biosynthesis was suppressed by JA inhibitors IBU and NDGA, which indicated that NO induced puerarin production through a JA-dependent signal pathway in the transgenic cells. Exogenous application of SA suppressed the elicitor-induced JA biosynthesis and reversed the inhibition of IBU and NDGA on elicitor-induced puerarin accumulation in transgenic cells, which indicated that SA inhibited JA biosynthesis in the cells and that SA might be used as a substitute for JA to mediate the elicitor- and NO-induced puerarin biosynthesis. It was, therefore, concluded that NO might mediate the elicitor-induced puerarin biosynthesis through SA- and JA-dependent signal pathways in wildtype P. thomsonii Benth. cells and transgenic NahG cells respectively.
Authors:
Maojun Xu; Jufang Dong; Muyuan Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Science in China. Series C, Life sciences / Chinese Academy of Sciences     Volume:  49     ISSN:  1006-9305     ISO Abbreviation:  Sci. China, C, Life Sci.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-22     Completed Date:  2006-11-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9611809     Medline TA:  Sci China C Life Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  379-89     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China. maojunxu@163.com
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cyclopentanes / metabolism*
Isoflavones / biosynthesis*
Nitric Oxide / physiology*
Oxylipins
Pueraria / metabolism*
Salicylic Acid / metabolism*
Signal Transduction / physiology*
Chemical
Reg. No./Substance:
0/Cyclopentanes; 0/Isoflavones; 0/Oxylipins; 10102-43-9/Nitric Oxide; 3681-99-0/puerarin; 6894-38-8/jasmonic acid; 69-72-7/Salicylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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