Document Detail

Nitric oxide mediates acute lung injury caused by fat embolism in isolated rat's lungs.
MedLine Citation:
PMID:  18301216     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The involvement of nitric oxide (NO) in acute lung injury (ALI) induced by fat embolism (FE) has not been investigated. The present study elucidated the role of NO in ALI because of FE. METHODS: FE was produced by introduction of fatty acid (corn oil micelles) into the isolated rat's lungs. Nonselective NO synthase (NOS) and selective inducible NOS (iNOS) inhibitors, N-nitro-l-arginine methyl ester (l-NAME) and l-N(1-iminoethyl)-lysine (l-Nil) as well as NO donors, sodium nitroprusside (SNP), and S-nitroso-N-acetylpenicillamine (SNAP) at a dose of 10 mol/L were given 60 minutes before FE. There were six groups of isolated lungs randomly assigned to receive vehicle (physiologic saline solution), FE, FE with pretreatment of l-NAME, l-Nil, SNP, or SNAP. Each group was observed for 4 hours. RESULTS: FE significantly increased the lung weight changes, pulmonary arterial pressure, and microvascular permeability. The concentration of nitrate or nitrite, methyl guanidine, tumor necrosis factor-alpha, and interleukin-1beta was significantly elevated after FE. Hisotopathologic examination revealed lung edema with multiple fatty droplets in lung tissue. Pretreatment with l-NAME or l-Nil attenuated, whereas SNP or SNAP exacerbated most of the FE-induced changes. Addition of NO donors (SNP or SNAP) into the isolated lungs did not produce significant changes in the lungs, suggesting that NO donation alone without FE does not exerts harmful effect. CONCLUSIONS: Our results suggest that NO production through the iNOS isoform plays a detrimental role in the FE-induced ALI. Free radical and proinflammatory cytokines may also be involved in the pathogenesis of ALI because of FE.
Shang-Jyh Kao; Hsing I Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of trauma     Volume:  64     ISSN:  1529-8809     ISO Abbreviation:  J Trauma     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-27     Completed Date:  2008-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376373     Medline TA:  J Trauma     Country:  United States    
Other Details:
Languages:  eng     Pagination:  462-9     Citation Subset:  AIM; IM    
Division of Chest Medicine, Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, and School of Respiratory Therapy, Taipei Medical University and Fu-Jen Catholic Medical College, Taipei, Taiwan.
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MeSH Terms
Body Weight
Bronchoalveolar Lavage Fluid / chemistry
Embolism, Fat / complications*,  pathology
Lung / metabolism,  pathology*
Nitric Oxide / metabolism*
Nitric Oxide Donors / metabolism
Organ Size
Pulmonary Embolism / complications*
Rats, Sprague-Dawley
Respiratory Distress Syndrome, Adult / etiology,  pathology
Reg. No./Substance:
0/Nitric Oxide Donors; 10102-43-9/Nitric Oxide

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