Document Detail


Nitric oxide is involved in light-specific responses of tomato during germination under normal and osmotic stress conditions.
MedLine Citation:
PMID:  22782244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: Nitric oxide (NO) is involved in the signalling and regulation of plant growth and development and responses to biotic and abiotic stresses. The photoperiod-sensitive mutant 7B-1 in tomato (Solanum lycopersicum) showing abscisic acid (ABA) overproduction and blue light (BL)-specific tolerance to osmotic stress represents a valuable model to study the interaction between light, hormones and stress signalling. The role of NO as a regulator of seed germination and ABA-dependent responses to osmotic stress was explored in wild-type and 7B-1 tomato under white light (WL) and BL.
METHODS: Germination data were obtained from the incubation of seeds on germinating media of different composition. Histochemical analysis of NO production in germinating seeds was performed by fluorescence microscopy using a cell-permeable NO probe, and endogenous ABA was analysed by mass spectrometry.
KEY RESULTS: The NO donor S-nitrosoglutathione stimulated seed germination, whereas the NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) had an inhibitory effect. Under WL in both genotypes, PTIO strongly suppressed germination stimulated by fluridone, an ABA inhibitor. The stimulatory effect of the NO donor was also observed under osmotic stress for 7B-1 seeds under WL and BL. Seed germination inhibited by osmotic stress was restored by fluridone under WL, but less so under BL, in both genotypes. This effect of fluridone was further modulated by the NO donor and NO scavenger, but only to a minor extent. Fluorescence microscopy using the cell-permeable NO probe DAF-FM DA (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate) revealed a higher level of NO in stressed 7B-1 compared with wild-type seeds.
CONCLUSIONS: As well as defective BL signalling, the differential NO-dependent responses of the 7B-1 mutant are probably associated with its high endogenous ABA concentration and related impact on hormonal cross-talk in germinating seeds. These data confirm that light-controlled seed germination and stress responses include NO-dependent signalling.
Authors:
Jana Piterková; Lenka Luhová; Jakub Hofman; Veronika Turecková; Ondrej Novák; Marek Petrivalsky; Martin Fellner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-09
Journal Detail:
Title:  Annals of botany     Volume:  110     ISSN:  1095-8290     ISO Abbreviation:  Ann. Bot.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-23     Completed Date:  2013-04-24     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  767-76     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University, Olomouc, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / metabolism
Cyclic N-Oxides / pharmacology
Fluoresceins / analysis
Free Radical Scavengers / pharmacology*
Gene Expression Regulation, Plant / drug effects,  radiation effects
Germination* / drug effects,  radiation effects
Imidazoles / pharmacology
Kinetics
Light*
Lycopersicon esculentum / drug effects,  genetics,  physiology*,  radiation effects
Models, Biological
Mutation
Nitric Oxide / pharmacology*
Nitric Oxide Donors / pharmacology
Osmosis / drug effects,  radiation effects
Plant Growth Regulators / metabolism
Pyridones / pharmacology
S-Nitrosoglutathione / pharmacology
Seeds / drug effects,  genetics,  physiology,  radiation effects
Signal Transduction / drug effects,  radiation effects
Stress, Physiological* / drug effects,  radiation effects
Chemical
Reg. No./Substance:
0/4-amino-5-methylamino-2',7'-difluorofluorescein diacetate; 0/Cyclic N-Oxides; 0/Fluoresceins; 0/Free Radical Scavengers; 0/Imidazoles; 0/Nitric Oxide Donors; 0/Plant Growth Regulators; 0/Pyridones; 10102-43-9/Nitric Oxide; 18390-00-6/2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; 21293-29-8/Abscisic Acid; 57564-91-7/S-Nitrosoglutathione; 59756-60-4/fluridone
Comments/Corrections

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