Document Detail


Nitric oxide inhibits the basic fibroblast growth factor-stimulated migration of bovine vascular smooth muscle cells in vitro.
MedLine Citation:
PMID:  10901085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The purpose of this study was to evaluate the effect of nitric oxide (NO) on basic fibroblast growth factor (bFGF)-stimulated migration of bovine VSMC in vitro.
METHODS: For the migration assay 50,000 cells from passages 4 to 8 were put into a migration chamber and stimulated with 10 ng bFGF/ml (control group). For another experiment, the NO donator sodium nitroprusside (SNP) (10(-6), 10(-8), 10(-10) M), the second messenger of NO 8-bromo-cGMP (10(-6), 10(-8), 10(-10) M) and 100 U of superoxide dismutase (SOD) were added. Migration of cells was quantified after fixing and staining of the cells by means of light microscopy.
RESULTS: Cell migration was significantly inhibited by SNP in a dose dependent manner (p < 0.01 with t-test). This effect was mirrored by 8-bromo-cGMP and not significantly inhibited by SOD, confirming this to be an NO mediated effect and excluding side-effects of superoxide-derived oxidation products.
CONCLUSION: Nitric oxide inhibits the basic fibroblast growth factor stimulated migration of bovine vascular smooth muscle cells in vitro.
Authors:
H Alfke; B Kleb; K J Klose
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  VASA. Zeitschrift für Gefässkrankheiten     Volume:  29     ISSN:  0301-1526     ISO Abbreviation:  VASA     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-09-26     Completed Date:  2000-09-26     Revised Date:  2012-10-19    
Medline Journal Info:
Nlm Unique ID:  0317051     Medline TA:  Vasa     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  99-102     Citation Subset:  IM    
Affiliation:
Department of Radiology, Philipps University of Marburg, Germany. alfke@mailer.uni-marburg.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Movement / drug effects*
Dose-Response Relationship, Drug
Fibroblast Growth Factor 2 / antagonists & inhibitors*,  pharmacology
Muscle, Smooth, Vascular / cytology*
Nitric Oxide / pharmacology*
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 103107-01-3/Fibroblast Growth Factor 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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