Document Detail


Nitric oxide induces heat shock protein 72 production and delayed protection against myocardial ischemia in rabbits via activating protein kinase C.
MedLine Citation:
PMID:  18706228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Nitric oxide (NO) is a biologically active molecule which has been reported to protect the heart against ischemia and reperfusion injury in different species. This study aimed to test the hypothesis that nitric oxide may induce the expression of heat shock protein 72 (HSP72) which may protect the heart against ischemia. METHODS: Rabbits were given intravenous saline or S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide donor, or Zaprinast, an inhibitor of cyclic guanosine monophosphate (GMP)-phosphodiesterase, which may increase myocardial cyclic GMP content. Twenty-four hours later, the rabbits were either sampled to measure HSP72, or induced with a 30-minute coronary occlusion followed by a 120-minute reperfusion, and then the infarct size was measured. Meanwhile, chelerythrine (CHE, an inhibitor of protein kinase C) was given intravenously 5 minutes before SNAP injection and the effect on HSP72 expression and infarct size was determined. RESULTS: Twenty-four hours after pretreatment, immunoblotting showed HSP72 expression increased in the SNAP group compared with control groups, and this was blocked by CHE. Myocardial infarct size in the SNAP group was smaller than that of the control group ((32.4 +/- 5.8)% vs (51.1 +/- 4.7)%, P < 0.05). Pretreated with CHE abolished the infarct size-limiting effect of SNAP ((46.0 +/- 5.1)%). Pretreatment with Zaprinast neither induced HSP72 expression nor reduced infarct size ((55.4 +/- 5.4)%). CONCLUSION: NO induced HSP72 expression and a delayed protection to the heart via the activities of protein kinase C by a cyclic GMP-independent pathway.
Authors:
Wei-jie Li; Zhi-jing Zhao; Bing Liu; Dian-xin Zhang; Fei Li; Hai-chang Wang; Wen-yi Guo; Guo-liang Jia; Masafumi Kitakaze; Masatsugu Hori
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  121     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-08-18     Completed Date:  2008-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  1109-13     Citation Subset:  IM    
Affiliation:
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710033, China. liweijie@fmmu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzophenanthridines / pharmacology
Cyclic GMP / metabolism
HSP72 Heat-Shock Proteins / biosynthesis*
Hemodynamics
Male
Myocardial Infarction / metabolism,  physiopathology,  prevention & control
Myocardial Ischemia / metabolism*,  physiopathology,  prevention & control
Nitric Oxide / metabolism*
Nitric Oxide Donors / pharmacology
Phosphodiesterase Inhibitors / pharmacology
Protein Kinase C / metabolism*
Purinones / pharmacology
Rabbits
S-Nitroso-N-Acetylpenicillamine / pharmacology
Chemical
Reg. No./Substance:
0/Benzophenanthridines; 0/HSP72 Heat-Shock Proteins; 0/Nitric Oxide Donors; 0/Phosphodiesterase Inhibitors; 0/Purinones; 10102-43-9/Nitric Oxide; 34316-15-9/chelerythrine; 37762-06-4/zaprinast; 7665-99-8/Cyclic GMP; 79032-48-7/S-Nitroso-N-Acetylpenicillamine; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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