| Nitric oxide has no obligatory role in isoflurane late preconditioning against myocardial stunning. | |
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MedLine Citation:
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PMID: 23044225 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIMS: Isoflurane has been demonstrated to produce late preconditioning against myocardial stunning. We tested the hypothesis that this effect is dependent upon an increased production of nitric oxide. MAIN METHODS: Studies were performed in 18 conscious dogs, chronically instrumented to measure coronary blood flow and myocardial wall thickening (WT). In Group 1 (control; n=7), a 10min coronary occlusion was produced followed by reperfusion; WT was monitored until full recovery. In Group 2 (n=6), the same occlusion-reperfusion protocol was performed 24h after inhalation of 1 MAC isoflurane (1.4 % in O(2)) for 60min. In Group 3 (n=5), the late anti-stunning effect of isoflurane was evaluated following non-selective inhibition of NOS with N-nitro-L-arginine (L-NA, 30mg/kg on three days beginning one day prior to isoflurane). Expression of eNOS and iNOS protein was measured by Western blotting. KEY FINDINGS: Two to three hours of reperfusion were required for recovery of WT following isoflurane (Group 2). In contrast, without isoflurane (Group 1), WT remained markedly reduced (30% below baseline) at this time point and required more than 6h of reperfusion for recovery. Treatment with L-NA (Group 3) did not alter time-course of recovery of WT following isoflurane. Isoflurane caused an increased expression of eNOS, but not of iNOS. SIGNIFICANCE: Isoflurane produced late preconditioning against myocardial stunning. Although this effect was associated with an upregulation of eNOS, its persistence following L-NA suggested that an increased production of nitric oxide did not play an obligatory role. |
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Authors:
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Song-Jung Kim; Gautam Malik; Maged M Saad; Sung-Ho Yoon; Joaquin B Gonzales; George J Crystal |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-5 |
Journal Detail:
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Title: Life sciences Volume: - ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Section of Cardiology, Advocate Illinois Masonic Medical Center, Chicago, IL; Department of Physiology and Biophysics, University of Illinois College Of Medicine, Chicago, IL. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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