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Nitric oxide has no obligatory role in isoflurane late preconditioning against myocardial stunning.
MedLine Citation:
PMID:  23044225     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: Isoflurane has been demonstrated to produce late preconditioning against myocardial stunning. We tested the hypothesis that this effect is dependent upon an increased production of nitric oxide. MAIN METHODS: Studies were performed in 18 conscious dogs, chronically instrumented to measure coronary blood flow and myocardial wall thickening (WT). In Group 1 (control; n=7), a 10min coronary occlusion was produced followed by reperfusion; WT was monitored until full recovery. In Group 2 (n=6), the same occlusion-reperfusion protocol was performed 24h after inhalation of 1 MAC isoflurane (1.4 % in O(2)) for 60min. In Group 3 (n=5), the late anti-stunning effect of isoflurane was evaluated following non-selective inhibition of NOS with N-nitro-L-arginine (L-NA, 30mg/kg on three days beginning one day prior to isoflurane). Expression of eNOS and iNOS protein was measured by Western blotting. KEY FINDINGS: Two to three hours of reperfusion were required for recovery of WT following isoflurane (Group 2). In contrast, without isoflurane (Group 1), WT remained markedly reduced (30% below baseline) at this time point and required more than 6h of reperfusion for recovery. Treatment with L-NA (Group 3) did not alter time-course of recovery of WT following isoflurane. Isoflurane caused an increased expression of eNOS, but not of iNOS. SIGNIFICANCE: Isoflurane produced late preconditioning against myocardial stunning. Although this effect was associated with an upregulation of eNOS, its persistence following L-NA suggested that an increased production of nitric oxide did not play an obligatory role.
Authors:
Song-Jung Kim; Gautam Malik; Maged M Saad; Sung-Ho Yoon; Joaquin B Gonzales; George J Crystal
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Life sciences     Volume:  -     ISSN:  1879-0631     ISO Abbreviation:  Life Sci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Section of Cardiology, Advocate Illinois Masonic Medical Center, Chicago, IL; Department of Physiology and Biophysics, University of Illinois College Of Medicine, Chicago, IL.
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