Document Detail

Nitric oxide-derived species in synovial fluid from patients with juvenile idiopathic arthritis.
MedLine Citation:
PMID:  15124263     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate superoxide anion (O2-), nitrite/nitrate (NO2-/NO3-), and nitrotyrosine (NT) production and the contribution of myeloperoxidase (MPO) to the production of NT-containing proteins in the synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA). The affected tissues in inflammatory arthritis produce large amounts of nitric oxide (NO) or peroxynitrite (ONOO-) but there are no reports of NO or ONOO- participation in JIA. We also attempted to correlate our findings with variables of disease activity and articular damage. METHODS: We analyzed 40 patients with JIA, mean age 12.7 years, mean disease duration 7.8 years. O2- production was measured by cytochrome C reduction after incubation of 106 synovial fluid (SF) cells with or without phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMLP) or opsonized zymosan. SF and serum NO2-/NO3- levels were measured by Griess reaction; NT was detected by Western blot. Myeloperoxidase (MPO) activity was estimated spectrophotometrically. Clinical and laboratory variables [erythrocyte sedimentation rate, C reactive protein (CRP), and radiological score] and interleukin 6 (IL-6) levels were evaluated. RESULTS: NO2-/NO3- production was greatly enhanced in the joints of JIA patients (54.6 +/- 3.2 micro M) when compared with serum (13.9 +/- 0.6 micro M; p < 0.001). NO2-/NO3- levels in SF were positively correlated with the number of infiltrating lymphomononuclear cells. NT-modified proteins detected in the SF showed a high correlation with radiological score, disease duration, CRP, and IL-6. CONCLUSION: Our results confirm the increased oxidative stress in children with JIA, suggesting a high in situ production of NO. The positive correlation between the expression of NT-modified proteins and variables of disease activity and damage is additional evidence that nitrogen and oxygen species may be involved in the joint destruction seen in patients with JIA.
Ana Paola N Lotito; Marcelo N Muscará; Maria Helena B Kiss; Simone A Teixeira; Gilberto S Novaes; Ieda Maria M Laurindo; Clovis A Silva; Suzana Beatriz V Mello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  31     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-04     Completed Date:  2004-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  992-7     Citation Subset:  IM    
Paediatric Division, Department of Internal Medicine, School of Medicine, Institute of Biomedical Sciences, University of São Paulo, Brazil.
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MeSH Terms
Arthritis, Juvenile Rheumatoid / metabolism*
Child, Preschool
Leukocytes, Mononuclear / pathology
Macrophage Activation / immunology
Nitric Oxide / metabolism
Peroxidase / metabolism
Reactive Nitrogen Species / metabolism*
Superoxides / metabolism
Synovial Fluid / cytology,  metabolism*
Tyrosine / analogs & derivatives*,  metabolism
Reg. No./Substance:
0/Reactive Nitrogen Species; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC

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