Document Detail


Nitric oxide-derived species in synovial fluid from patients with juvenile idiopathic arthritis.
MedLine Citation:
PMID:  15124263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate superoxide anion (O2-), nitrite/nitrate (NO2-/NO3-), and nitrotyrosine (NT) production and the contribution of myeloperoxidase (MPO) to the production of NT-containing proteins in the synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA). The affected tissues in inflammatory arthritis produce large amounts of nitric oxide (NO) or peroxynitrite (ONOO-) but there are no reports of NO or ONOO- participation in JIA. We also attempted to correlate our findings with variables of disease activity and articular damage. METHODS: We analyzed 40 patients with JIA, mean age 12.7 years, mean disease duration 7.8 years. O2- production was measured by cytochrome C reduction after incubation of 106 synovial fluid (SF) cells with or without phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMLP) or opsonized zymosan. SF and serum NO2-/NO3- levels were measured by Griess reaction; NT was detected by Western blot. Myeloperoxidase (MPO) activity was estimated spectrophotometrically. Clinical and laboratory variables [erythrocyte sedimentation rate, C reactive protein (CRP), and radiological score] and interleukin 6 (IL-6) levels were evaluated. RESULTS: NO2-/NO3- production was greatly enhanced in the joints of JIA patients (54.6 +/- 3.2 micro M) when compared with serum (13.9 +/- 0.6 micro M; p < 0.001). NO2-/NO3- levels in SF were positively correlated with the number of infiltrating lymphomononuclear cells. NT-modified proteins detected in the SF showed a high correlation with radiological score, disease duration, CRP, and IL-6. CONCLUSION: Our results confirm the increased oxidative stress in children with JIA, suggesting a high in situ production of NO. The positive correlation between the expression of NT-modified proteins and variables of disease activity and damage is additional evidence that nitrogen and oxygen species may be involved in the joint destruction seen in patients with JIA.
Authors:
Ana Paola N Lotito; Marcelo N Muscará; Maria Helena B Kiss; Simone A Teixeira; Gilberto S Novaes; Ieda Maria M Laurindo; Clovis A Silva; Suzana Beatriz V Mello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  31     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-04     Completed Date:  2004-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  992-7     Citation Subset:  IM    
Affiliation:
Paediatric Division, Department of Internal Medicine, School of Medicine, Institute of Biomedical Sciences, University of São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Arthritis, Juvenile Rheumatoid / metabolism*
Child
Child, Preschool
Female
Humans
Leukocytes, Mononuclear / pathology
Macrophage Activation / immunology
Male
Nitric Oxide / metabolism
Peroxidase / metabolism
Reactive Nitrogen Species / metabolism*
Superoxides / metabolism
Synovial Fluid / cytology,  metabolism*
Tyrosine / analogs & derivatives*,  metabolism
Chemical
Reg. No./Substance:
0/Reactive Nitrogen Species; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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