Document Detail

Nitric oxide-dependent bradycardia in mutant analbuminemic rats.
MedLine Citation:
PMID:  22889827     Owner:  NLM     Status:  MEDLINE    
Nagase analbuminemic rats (NAR) are natural mutant Sprague-Dawley rats which do not express albumin due to a single splice mutation in the albumin gene. We accidentally discovered that NAR have a significant bradycardia compared with wild type Sprague-Dawley rats, and the present study was carried out to investigate the mechanism of bradycardia in analbuminemic rats. In vitro studies showed that the basal spontaneous beating rate of isolated atria is similar in NAR compared with wild type animals. However, the chronotropic responsiveness of isolated atria to cholinergic stimulation was markedly increased in NAR, an effect which was prevented by incubation with a nitric oxide synthase (NOS) or guanylyl cyclase inhibitor. NAR had a significant increase in plasma nitrite/nitrate concentrations. Administration of a NOS inhibitor for 5 days normalized heart rate in NAR. The level of NOS isoforms, caveolin-1 and caveolin-3 expression in the atria was assessed by real time PCR. There was no significant difference in the expression of NOS isoforms or caveolin-3 in NAR compared with wild type controls. However, NAR exhibited a significant decrease in caveolin-1 expression in the atria. Since caveolin-1 is known to inhibit endothelial NOS activity in cardiomyocytes, we suggest that decreased caveolin-1 levels may have a role in increased nitric oxide production in NAR. Our data suggest that a NOS/cGMP-dependent mechanism might be involved in increased responsiveness to vagal stimulation and bradycardia in analbuminemic condition.
Ali R Mani; Silvia Ippolito; Miguel N Centelles; Kevin P Moore
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Publication Detail:
Type:  Journal Article     Date:  2012-08-11
Journal Detail:
Title:  Biochemical pharmacology     Volume:  84     ISSN:  1873-2968     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-14     Completed Date:  2012-12-13     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1062-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Albumins / analysis*
Base Sequence
Blotting, Western
Bradycardia / physiopathology*
Caveolin 1 / metabolism
Caveolin 3 / metabolism
DNA Primers
Enzyme Inhibitors / pharmacology
Heart Rate
Microscopy, Immunoelectron
Nitric Oxide / physiology*
Nitric Oxide Synthase / antagonists & inhibitors,  metabolism
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Grant Support
G0800971//Medical Research Council
Reg. No./Substance:
0/Albumins; 0/Caveolin 1; 0/Caveolin 3; 0/DNA Primers; 0/Enzyme Inhibitors; 31C4KY9ESH/Nitric Oxide; EC Oxide Synthase

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