Document Detail

Nitric Oxide contributes to the regulation of iron metabolism in skeletal muscle in vivo and in vitro.
MedLine Citation:
PMID:  20411304     Owner:  NLM     Status:  MEDLINE    
Nitric Oxide (NO) plays an important role in iron redistribution during exercise, while its molecular regulatory mechanism is still not clear. Our present studies were to investigate the effects of NO on iron metabolism and to elucidate the regulatory mechanism of iron transport in skeletal muscle both in vivo and in vitro. One group of male Wistar rats (300 +/- 10 g) were subjected to an exercise of 30 min on a treadmill for 5 weeks (exercise group, EG, 6 rats) and the other one was placed on the treadmill without running (control group, CG, 6 rats). The cultured L6 rat skeletal muscle cells were treated with either 0.5 mM SNAP (NO donor) or not for 24 h, and their iron release and intake amount were examined by measuring radiolabelled (55)Fe. The results showed: (1) The NO content (CG, 1.09 +/- 0.18 micromol/g vs. EG, 1.49 +/- 0.17 micromol/g) and non-heme iron in gastrocnemius (CG, 118.35 +/- 11.41 microg/g vs. EG, 216.65 +/- 11.10 microg/g) of EG were significantly increased compared with CG. (2) The expression of DMT1 (IRE) and TfR1 of EG was increased. (3) The iron intake was increased in L6 cells treated with SNAP (P < 0.01). (4) Western blot results showed the protein level of both TfR1 and DMT1 (IRE) in SNAP cells were up-regulated, while the expression of FPN1 was down-regulated (P < 0.05). The data suggested that the induced elevation of NO level by exercise lead to the up-regulation of both TfR1 and DMT1 (IRE), which in turn increasing the iron absorption in skeletal muscle.
Haitao Wang; Xianglin Duan; Jianguo Liu; Huanbin Zhao; Yuqian Liu; Yanzhong Chang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-22
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  342     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-18     Completed Date:  2011-01-04     Revised Date:  2014-04-10    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  87-94     Citation Subset:  IM    
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MeSH Terms
Blotting, Western
Cation Transport Proteins / metabolism
Cells, Cultured
Free Radical Scavengers / pharmacology*
Iron / metabolism*
Muscle, Skeletal / cytology,  drug effects*,  metabolism
Myoglobin / metabolism
Nitric Oxide / pharmacology*
Physical Conditioning, Animal
Rats, Wistar
Receptors, Transferrin / metabolism
Succinate Dehydrogenase / drug effects,  metabolism
Reg. No./Substance:
0/Cation Transport Proteins; 0/Free Radical Scavengers; 0/Myoglobin; 0/Receptors, Transferrin; 0/Tfr1 protein, rat; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 31C4KY9ESH/Nitric Oxide; E1UOL152H7/Iron; EC Dehydrogenase

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