Document Detail

Nitric oxide and cGMP do not affect fluid flux in isolated rat lungs.
MedLine Citation:
PMID:  8847334     Owner:  NLM     Status:  MEDLINE    
We sought to examine the influence of nitric oxide (NO) and the second messengers guanosine 3',5'-cyclic monophosphate (cGMP) and intracellular Ca2+ on fluid flux in lungs isolated from male Sprague-Dawley rats and perfused with saline (containing 4% albumin) or with whole blood. Lungs were allowed to equilibrate for a period of 30 min without treatment (control group) or with one of the following agents: the exogenous NO donor spermine NONOate, the nitric oxide synthase inhibitor N omega-nitro-L-arginine (L-NNA), 8-BrcGMP, the Ca2- ionophore ionomycin, or the endothelial injurious agent protamine. After equilibration, perfusate reservoir height was increased to five incremental settings to increase pulmonary venous pressure and enhance fluid flux. Perfusate reservoir weight was monitored continuously as an index of fluid flux. The lung wet-to-dry weight ratio was determined on completion of the experiments. Increasing reservoir height was associated with an increase in pulmonary arterial, pulmonary capillary, and pulmonary venous pressures and an increase in fluid flux. However, treatment with exogenous NO or inhibition of endogenous NO was without effect on fluid flux in saline lungs at two different flow rates or in whole blood-perfused lungs. Similarly, treatment with cGMP and ionomycin did not alter fluid flux. Protamine pretreatment resulted in a significant increase in fluid flux at the highest reservoir setting, although exogenous NO and L-NNA pretreatments were without further effect on the protamine-treated lungs. Thus a role for NO and the second messengers cGMP and Ca2+ in modulating fluid flux could not be demonstrated in the isolated rat lung.
M R Eichinger; B R Walker
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  80     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-10-24     Completed Date:  1996-10-24     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  69-76     Citation Subset:  IM    
Department of Physiology, University of New Mexico School of Medicine, Albuquerque 87131. USA.
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MeSH Terms
Arginine / analogs & derivatives,  pharmacology
Blood Pressure / drug effects
Body Fluids / physiology*
Calcium / pharmacology
Cyclic GMP / analogs & derivatives,  pharmacology*
Enzyme Inhibitors / pharmacology
Heparin Antagonists / toxicity
Ionomycin / diagnostic use
Lung / drug effects*
Nitric Oxide / metabolism,  pharmacology*
Nitric Oxide Synthase / antagonists & inhibitors
Nitrogen Oxides
Organ Size / physiology
Protamines / toxicity
Pulmonary Circulation / drug effects
Rats, Sprague-Dawley
Spermine / analogs & derivatives,  pharmacology
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Heparin Antagonists; 0/Nitrogen Oxides; 0/Protamines; 10102-43-9/Nitric Oxide; 136587-13-8/spermine nitric oxide complex; 2149-70-4/Nitroarginine; 31356-94-2/8-bromocyclic GMP; 56092-81-0/Ionomycin; 71-44-3/Spermine; 74-79-3/Arginine; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; EC Oxide Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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