| Nitric oxide blocks the cell cycle of mouse macrophage-like cells in the early G2+M phase. | |
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MedLine Citation:
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PMID: 8131837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of nitric oxide produced by macrophage-like cells (Mm1) on the cell cycle were investigated. Mm1 cells lost proliferative activity in the presence of interleukin-6 (IL-6) and a subpopulation accumulated in the G2+M phase. This level increased in proportion to the incubation time. The DNA content of the cells was slightly lower than that of Mm1 cells treated with vinblastine or demecolcine, drugs which block the cell cycle in the M phase. The peak of the early G2+M phase was reduced by treatment with NG-mono-methyl-L-arginine. However, after treatment with exogenous nitric oxide or sodium nitroprusside, the G0/G1 phase increased, but the early-G2+M and the S phase decreased. The flow cytometry pattern in IL-6-treated Mm1 was the same as that of cytochalasin B-treated Mm1. These data suggest that endogenous nitric oxide affects the microfilament system of IL-6-treated Mm1 cells and blocks the cell cycle in the early G2+M phase. |
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Authors:
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K Takagi; Y Isobe; K Yasukawa; E Okouchi; Y Suketa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEBS letters Volume: 340 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1994 Mar |
Date Detail:
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Created Date: 1994-04-15 Completed Date: 1994-04-15 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 159-62 Citation Subset: IM |
Affiliation:
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Department of Environmental Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / drug effects* Cell Line DNA / metabolism G2 Phase / drug effects Interleukin-6 / pharmacology Macrophages / cytology, drug effects*, metabolism Mice Mitosis / drug effects Nitric Oxide / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6; 10102-43-9/Nitric Oxide; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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