Document Detail

Nitric oxide blocks the cell cycle of mouse macrophage-like cells in the early G2+M phase.
MedLine Citation:
PMID:  8131837     Owner:  NLM     Status:  MEDLINE    
The effects of nitric oxide produced by macrophage-like cells (Mm1) on the cell cycle were investigated. Mm1 cells lost proliferative activity in the presence of interleukin-6 (IL-6) and a subpopulation accumulated in the G2+M phase. This level increased in proportion to the incubation time. The DNA content of the cells was slightly lower than that of Mm1 cells treated with vinblastine or demecolcine, drugs which block the cell cycle in the M phase. The peak of the early G2+M phase was reduced by treatment with NG-mono-methyl-L-arginine. However, after treatment with exogenous nitric oxide or sodium nitroprusside, the G0/G1 phase increased, but the early-G2+M and the S phase decreased. The flow cytometry pattern in IL-6-treated Mm1 was the same as that of cytochalasin B-treated Mm1. These data suggest that endogenous nitric oxide affects the microfilament system of IL-6-treated Mm1 cells and blocks the cell cycle in the early G2+M phase.
K Takagi; Y Isobe; K Yasukawa; E Okouchi; Y Suketa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  340     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-04-15     Completed Date:  1994-04-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  159-62     Citation Subset:  IM    
Department of Environmental Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Japan.
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MeSH Terms
Cell Cycle / drug effects*
Cell Line
DNA / metabolism
G2 Phase / drug effects
Interleukin-6 / pharmacology
Macrophages / cytology,  drug effects*,  metabolism
Mitosis / drug effects
Nitric Oxide / pharmacology*
Reg. No./Substance:
0/Interleukin-6; 10102-43-9/Nitric Oxide; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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