Document Detail


Nitric oxide biomarkers increase during exercise-induced vasodilation in the forearm.
MedLine Citation:
PMID:  10727066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the study was to determine if exercise-induced vasodilation was associated with an increase in forearm plasma levels of nitric oxide (NO) biomarkers (NO2- + NO3- and L-citrulline). Twelve healthy subjects (27+/-6 yrs) performed incremental rhythmic forearm exercise with the nondominant hand for 6 min each at 15, 30 and 45% of maximal voluntary contraction (MVC). Forearm blood flow (FBF) was determined in the exercise arm using venous occlusion plethysmography. Blood samples were obtained from the antecubital vein of the exercise and nonexercise arms for the measurement of NO biomarkers. In the exercise arm, FBF increased by a mean of 150%, 335% and 585% above baseline at 15, 30 and 45% of MVC, respectively. (ANOVA, P= 0.0001). Venous plasma NO2- + NO3- levels increased from 24+/-4 micromol/L at baseline, to 29+/-5, 32+/-4 and 3+/-4 micromol/L (ANOVA, P = 0.0001). Venous plasma L-citrulline levels increased from 31+/-5 micromol/L at baseline to 58+/-10, 87+/-7 and 141+/-15 micromol/L (ANOVA, P = 0.0001). There was a linear relationship between FBF and venous plasma NO2- + NO3- (slope= 0.38+/-0.10, P=0.0007) and between L-citrulline, (slope= 5.1 +/-1.3, P = 0.0004). Venous plasma levels of NO2- + NO3- and L-citrulline in the nonexercise arm were unchanged. These results demonstrate that exercise-induced vasodilation in the forearm is associated with forearm plasma levels of NO2- + NO3- and L-citrulline, in vivo markers of NO production.
Authors:
M D Brown; M Srinivasan; R V Hogikyan; D R Dengel; S G Glickman; A Galecki; M A Supiano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of sports medicine     Volume:  21     ISSN:  0172-4622     ISO Abbreviation:  Int J Sports Med     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-04-21     Completed Date:  2000-04-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008349     Medline TA:  Int J Sports Med     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  83-9     Citation Subset:  IM; S    
Affiliation:
Department of Internal Medicine, University of Michigan, Ann Arbor, USA. mb166@umail.umd.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / analysis
Exercise / physiology*
Female
Forearm
Humans
Male
Muscle, Skeletal / physiology
Nitric Oxide / blood*
Sensitivity and Specificity
Vasodilation / physiology*
Grant Support
ID/Acronym/Agency:
AG08808/AG/NIA NIH HHS; KO 1 AG0072301/AG/NIA NIH HHS; T32 AG00114/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 10102-43-9/Nitric Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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