Document Detail


Nitric oxide as a mediator of reduced arterial responsiveness in septic patients.
MedLine Citation:
PMID:  8681578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the functional status of arteries in patients with septic shock, each of whom suffered from severe hypotension. DESIGN: Experimental, comparative study. SETTING: Laboratory in a university hospital. SUBJECTS: Mesenteric artery resected from omentum was obtained from patients (n = 3) with or without (n = 4) sepsis. INTERVENTIONS: To study the effect of modification of the nitric oxide system in human arteries during sepsis, changes in norepinephrine-evoked isometric tension in mesenteric arterial rings were measured. MEASUREMENTS AND MAIN RESULTS: Mesenteric arteries were isolated from omentum resected from three patients with septic shock and from four patients with no inflammatory conditions; in each case, during an intestinal anastomosis. In arterial rings, after a 1-hr equilibration with Krebs solution, changes in isometric tension evoked by norepinephrine were measured for 5 mins every 20 mins. The tension initially evoked in rings from septic patients was lower than in those rings from nonseptic patients and, unlike the controls, it decreased with a short time course. The addition of 300 micromol/L of N(G)-nitro-L-arginine methyl ester or 10 micromol of N(G)-nitro-L-arginine methyl ester or 10 micromol/L of methylene blue reversed this decrease. CONCLUSION: These results indicate that in patients with septic shock, the main cause of reduced sensitivity to pressor agents may be a massive generation of nitric oxide via the L-arginine pathway.
Authors:
I Tsuneyoshi; Y Kanmura; N Yoshimura
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Critical care medicine     Volume:  24     ISSN:  0090-3493     ISO Abbreviation:  Crit. Care Med.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-21     Completed Date:  1996-08-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0355501     Medline TA:  Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1083-6     Citation Subset:  AIM; IM    
Affiliation:
Department of Anesthesiology and Critical Care Medicine, Kagoshima University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aged
Arginine / analogs & derivatives,  pharmacology
Enzyme Inhibitors / pharmacology
Female
Hemodynamics
Humans
Hypotension / complications,  etiology
Mesenteric Arteries / drug effects*
Middle Aged
NG-Nitroarginine Methyl Ester
Nitric Oxide / biosynthesis*
Norepinephrine / pharmacology*
Shock, Septic / complications,  etiology,  physiopathology*
Vasoconstriction / drug effects
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-41-2/Norepinephrine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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