Document Detail


Nitric oxide-dependent pigment migration induced by ultraviolet radiation in retinal pigment cells of the crab Neohelice granulata.
MedLine Citation:
PMID:  21091482     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The purpose of this study was to verify the occurrence of pigment dispersion in retinal pigment cells exposed to UVA and UVB radiation, and to investigate the possible participation of a nitric oxide (NO) pathway. Retinal pigment cells from Neohelice granulata were obtained by cellular dissociation. Cells were analyzed for 30 min in the dark (control) and then exposed to 1.1 and 3.3 J cm(-2) UVA, 0.07 and 0.9 J cm(-2) UVB, 20 nmβ-PDH (pigment dispersing hormone) or 10 μm SIN-1 (NO donor). Histological analyses were performed to verify the UV effect in vivo. Cultured cells were exposed to 250 μm L-NAME (NO synthase blocker) and afterwards were treated with UVA, UVB or β-PDH. The retinal cells in culture displayed significant pigment dispersion in response to UVA, UVB and β-PDH. The same responses to UVA and UVB were observed in vivo. SIN-1 did not induce pigment dispersion in the cell cultures. L-NAME significantly decreased the pigment dispersion induced by UVA and UVB but not by β-PDH. All retinal cells showed an immunopositive reaction against neuronal nitric oxide synthases. Therefore, UVA and UVB radiation are capable of inducing pigment dispersion in retinal pigment cells of Neohelice granulata and this dispersion may be nitric oxide synthase dependent.
Authors:
Daza de Moraes Vaz Batista Filgueira; Laís Pereira Guterres; Ana Paula de Souza Votto; Marcelo Alves Vargas; Robert Tew Boyle; Gilma Santos Trindade; Luiz Eduardo Maia Nery
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  86     ISSN:  1751-1097     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1278-84     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal Compilation. The American Society of Photobiology.
Affiliation:
Programa de Pós-graduação em Ciências Fisiológicas, Fisiologia Animal Comparada, FURG, Instituto de Ciências Biológicas, Rio Grande, Brazil.
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