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Nitric Oxide Up-regulates the Expression of Methionine Sulfoxide Reductase Genes in the Intertidal Macroalga Ulva fasciata for High Light Acclimation.
MedLine Citation:
PMID:  22210901     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Nitric oxide (NO) has emerged as a fundamental signal molecule involving in the responses of plant to stress. A role of NO in the regulation of methionine sulfoxide reductase (MSR) mRNA expression and high light acclimation was studied in a green macroalga Ulva fasciata Delile. A transfer from darkness to high light (≥ 1,200 μmol photons·m(-2)·s(-1)) inhibited photosynthesis and growth but increased NO production and UfMSRA and UfMSRB transcripts. Trreatment of an NO scavenger, 2-(4-carboxy-phenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (cPTIO), in 1,200 μmol photons·m(-2)·s(-1) condition caused a further growth inhibition accompanied by an inhibition of the increase of UfMSRA and UfMSRB transcripts by high light, while the treatment of an NO generator, sodium nitroprusside (SNP), alleviated growth inhibition and enhanced UfMSRA and UfMSRB expression. An exposure to moderate light (300 μmol photons·m(-2)·s(-1)) condition also increased UfMSRA and UfMSRB transcripts, which were not affected by cPTIO treatment but enhanced by SNP treatment. So, NO does not mediate the up-regulation of UfMSR by a transfer to moderate light possibly as a pre-caution mechanism in the sense of increasing light intensities in the daytime. In conclusion, NO production can be induced in U. fasciata upon exposure to high light for up-regulation of UfMSRA and UfMSRB expression but the level of NO production is not sufficient for acquisition of full tolerance to high light stress. Enhanced NO production by exogenously applied NO generator can effectively trigger high light acclimation process, including UfMSRA and UfMSRB expression.
Authors:
Yuan-Ting Hsu; Tse-Min Lee
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-30
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
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