| Nitric Oxide Production Induced in Roots of Lotus japonicus by Lipopolysaccharide from Mesorhizobium loti. | |
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MedLine Citation:
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PMID: 21330297 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Lipopolysaccharide (LPS) is a bacterial molecule that induces nitric oxide (NO) production and triggers defense systems in plant-pathogen interactions. NO production is induced in the roots of Lotus japonicus after inoculation of the roots with its microsymbiont Mesorhizobium loti. However, the rhizobial molecule that induces NO production has not yet been identified. We investigated NO production in the roots of L. japonicus by treatment with LPS of M. loti. LPS was prepared by phenol-hot water extraction and separated into several fractions: polysaccharide, lipooligosaccharide, oligosaccharide, and lipid A. In the roots of L. japonicus, NO production was observed with NO-specific fluorescent dye 4, 10, and 24 h after treatment with each fraction of LPS. NO production was detected 4 h after treatment with all fractions. NO production was also detectable 24 h after treatment, except after treatment of the polysaccharide and oligosaccharide fractions. Expression of a class1 hemoglobin gene and application of an NO scavenger showed the treatment of LPS and LOS induced the similar response to inoculation with M.loti. These data suggest that LPS of M. loti induces NO production after inoculation with M. loti. |
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Authors:
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Ei-Ichi Murakami; Maki Nagata; Yoshikazu Shimoda; Ken-Ichi Kucho; Shiro Higashi; Mikiko Abe; Masahito Hashimoto; Toshiki Uchiumi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-16 |
Journal Detail:
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Title: Plant & cell physiology Volume: - ISSN: 1471-9053 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Graduate School of Science and Engineering, Kagoshima University, Kagoshima, 890-0065 Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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