Document Detail

Nitration of lysozyme by ultrasonic waves; demonstration by immunochemistry and mass spectrometry.
MedLine Citation:
PMID:  20667761     Owner:  NLM     Status:  MEDLINE    
Solutions containing hen egg white lysozyme (HEWL) and nitrite were exposed to ultrasonic irradiation in order to study the possible sonochemical modifications. This is the first demonstration of the nitration of tyrosine residues in a protein (lysozyme) by the use of an ultrasonic field alone. Sonochemically nitrated lysozyme was detected using the immunochemical techniques dot blot immunodetection and enzyme-linked immunosorbent assay (ELISA). The sonically oxidised and nitrated protein solutions were analysed by Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Hydroxylated species were found in the absence of nitrite, whereas nitration was the major modification in the presence of nitrating agent, implying a competing mechanism between hydroxyl radicals and nitrite. Circular dichroism (CD) indicated that the ultrasonic experimental conditions chosen in this study had little effect on the tertiary and secondary structures of HEWL. Whilst enzymatic assay showed that the presence of nitrite provided a protective effect on the inactivation of the protein under ultrasonic irradiation, nevertheless partially purified, sonically nitrated lysozyme showed a dramatic decrease in lytic activity.
Sadie Dean; Martin Cox; John Heptinstall; David J Walton; Victor A Mikhailov; Helen J Cooper; María Gómez-Mingot; Jesús Iniesta
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-27
Journal Detail:
Title:  Ultrasonics sonochemistry     Volume:  18     ISSN:  1873-2828     ISO Abbreviation:  Ultrason Sonochem     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-09-13     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9433356     Medline TA:  Ultrason Sonochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  334-44     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Department of Biomolecular and Sport Science, Coventry University, Coventry CV1 5FB, United Kingdom.
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MeSH Terms
Egg White / chemistry*
Mass Spectrometry
Muramidase / chemistry*,  metabolism
Nitrites / chemistry*
Reg. No./Substance:
0/Nitrites; EC

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