Document Detail


Nitrated fatty acids: Synthesis and measurement.
MedLine Citation:
PMID:  23200809     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis, sample extraction from complex biological matrices, and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by LC-MS. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.
Authors:
Steven R Woodcock; Gustavo Bonacci; Stacy L Gelhaus; Francisco J Schopfer
Related Documents :
20420369 - Polymethoxylated flavones, flavanone glycosides, carotenoids, and antioxidants in diffe...
1229719 - The phenolics of fruits. vii. the phenolics of cherries and plums and the changes in ca...
11714229 - Bagremycin a and b, novel antibiotics from streptomyces sp. tü 4128.
16227639 - Lipoic acid - the drug of the future?
18608569 - Release of antioxidant components from tomatoes determined by an in vitro digestion met...
24242839 - Characterization of diacylglycerylphosphocholine molecular species by fab-cad-ms/ms: a ...
6233389 - The simultaneous electrophoretic analysis of esterase d and phosphoglucomutase subtypin...
17951499 - Carbohydrate digestibility and metabolic effects.
4083549 - Comparative electrophoretic study between rabbit and human seminal fluid.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  Free radical biology & medicine     Volume:  -     ISSN:  1873-4596     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Department of Pharmacology & Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bosentan inhibits oxidative and nitrosative stress and rescues occlusive pulmonary hypertension.
Next Document:  Modelling and Analysing the Dynamics of Disease Progression from Cross-Sectional Studies.