Document Detail

Nitrate and nitrite anion concentration in the intact cerebral cortex of preterm and nearterm fetal sheep: indirect index of in vivo nitric oxide formation.
MedLine Citation:
PMID:  9741385     Owner:  NLM     Status:  MEDLINE    
Pregnant sheep with a microdialysis probe implanted in the fetal cerebral cortex were used to determine if nitrate and nitrite anions (nitrate/nitrite) could be quantitated in the microdialysate as an indirect index of in vivo nitric oxide formation. Pregnant ewes (term, about 147 days) were surgically instrumented at gestational day (GD) 90 (n = 3; preterm) and GD 121 (n = 3; nearterm). Three days later, following an overnight probe equilibration period, five dialysate samples were collected continuously on ice at 1-h intervals (infusion rate of 1 (microl/min). The nitrate/nitrite concentration was determined by reducing a 10-microl aliquot of each dialysate fraction with hot acidic vanadium followed by chemiluminescence quantitation of the nitric oxide product. The lower limit of quantitative sensitivity of the method is 25 picomoles. Nitrate/nitrite concentration was 16.6+/-7.3 microM for the preterm fetus and 19.7+/-1.9 microM for the nearterm fetus. The data demonstrate that nitrate/nitrite, as an index of in vivo nitric oxide formation, can be quantitated in microdialysate samples collected from the intact fetal sheep cerebral cortex.
J D Reynolds; G A Zeballos; D H Penning; K A Kimura; B Atkins; J F Brien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  39     ISSN:  1056-8719     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-11-20     Completed Date:  1998-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  125-8     Citation Subset:  IM    
Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
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MeSH Terms
Cerebral Cortex / embryology*,  metabolism*
Nitrates / metabolism*
Nitric Oxide / biosynthesis*
Nitrites / metabolism*
Reg. No./Substance:
0/Anions; 0/Nitrates; 0/Nitrites; 10102-43-9/Nitric Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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