| Nitrate reductase activity of mitochondrial aldehyde dehydrogenase (ALDH-2) as a redox sensor for cardiovascular oxidative stress. | |
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MedLine Citation:
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PMID: 20072908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In 2002, mitochondrial aldehyde dehydrogenase (ALDH-2) was identified as an organic nitrate bioactivating enzyme. This so-called nitrate reductase activity denitrates nitroglycerin (glycerol trinitrate) to its 1,2-glycerol dinitrate metabolite and nitrite. This reaction relies on reduced thiols at the active site of the enzyme and on the presence of reduced dithiols as the electron source. During bioconversion of nitroglycerin, and also in the presence of reactive oxygen and nitrogen species, the active site thiols of ALDH-2 are oxidized and the enzyme looses its activity. We, therefore, speculated that ALDH-2 activity could be a useful marker for cardiovascular oxidative stress. Indeed, this hypothesis was supported by a number of studies, indicating that ALDH-2 activity is impaired in experimental animal models of increased oxidative stress and may be used for detection of an imbalance of mitochondrial and cellular redox state. |
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Authors:
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Andreas Daiber; Thomas M?nzel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 594 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 43-55 Citation Subset: IM |
Affiliation:
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II. Medizinische Klinik - Labor f?r Molekulare Kadiologie, Johannes Gutenberg-Universit?t, Mainz, Germany. daiber@uni-mainz.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Dehydrogenase
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metabolism* Animals Cardiovascular System / metabolism* Humans Mitochondrial Proteins / metabolism* Models, Biological Oxidation-Reduction Oxidative Stress / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Mitochondrial Proteins; EC 1.2.1.3/Aldehyde Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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