Document Detail


Nipah virus entry can occur by macropinocytosis.
MedLine Citation:
PMID:  19854459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nipah virus (NiV) is a zoonotic biosafety level 4 paramyxovirus that emerged recently in Asia with high mortality in man. NiV is a member, with Hendra virus (HeV), of the Henipavirus genus in the Paramyxoviridae family. Although NiV entry, like that of other paramyxoviruses, is believed to occur via pH-independent fusion with the host cell's plasma membrane we present evidence that entry can occur by an endocytic pathway. The NiV receptor ephrinB2 has receptor kinase activity and we find that ephrinB2's cytoplasmic domain is required for entry but is dispensable for post-entry viral spread. The mutation of a single tyrosine residue (Y304F) in ephrinB2's cytoplasmic tail abrogates NiV entry. Moreover, our results show that NiV entry is inhibited by constructions and drugs specific for the endocytic pathway of macropinocytosis. Our findings could potentially permit the rapid development of novel low-cost antiviral treatments not only for NiV but also HeV.
Authors:
Olivier Pernet; Christine Pohl; Michelle Ainouze; Hasan Kweder; Robin Buckland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-24
Journal Detail:
Title:  Virology     Volume:  395     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-27     Completed Date:  2009-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  298-311     Citation Subset:  IM    
Affiliation:
Molecular Basis of Paramyxovirus Entry, INSERM U758 Virologie Humaine IFR 128 BioSciences Gerland-Lyon Sud, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07, France.
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / antagonists & inhibitors
Animals
CHO Cells
Cricetinae
Cricetulus
Hydrogen-Ion Concentration
Nipah Virus / physiology*
Recombinant Proteins
Viral Proteins / genetics,  metabolism
Virus Internalization / drug effects*
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/Viral Proteins; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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