| Nipah virus conforms to the rule of six in a minigenome replication assay. | |
| | |
MedLine Citation:
|
PMID: 14993656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To study the replication of Nipah virus (NiV), a minigenome replication assay that does not require the use of infectious virus was developed. The minigenome was constructed to encode a NiV vRNA analogue containing the gene for chloramphenicol acetyltransferase (CAT) under the control of putative NiV transcription motifs and flanked by the NiV genomic termini. CAT protein was detected only when plasmids encoding the NiV minigenome, nucleocapsid protein (N), phosphoprotein (P) and polymerase protein (L) were transfected into CV1 cells. To determine whether NiV conforms to the rule of six, a series of plasmids encoding minigenomes that differed in length by a single nucleotide was tested in the replication assay. CAT production was detected only with the minigenome whose length was an even multiple of six. The replication assay was also used to show that the N, P and L proteins of NiV recognize cis-acting sequences in the genomic termini of Hendra virus (HeV) but not measles virus. While these results suggest that NiV uses a replication strategy that is similar to those of other paramyxoviruses, they also support the inclusion of NiV and HeV in a separate genus within the subfamily Paramyxovirinae. |
| | |
Authors:
|
Kim Halpin; Bettina Bankamp; Brian H Harcourt; William J Bellini; Paul A Rota |
Related Documents
:
|
6409836 - Evidence for a virus in non-a, non-b hepatitis transmitted via the fecal-oral route. 11296746 - Duplex rt-pcr for simultaneous detection of hepatitis a and hepatitis e virus isolated ... 6271006 - Pathogenetic aspects of hepatitis a virus infection in enterally inoculated marmosets. 6316896 - Role of splenectomy in prevention of hemorrhagic enteritis and death from hemorrhagic e... 17454446 - Influenza a virus transactivates the mouse envelope gene encoding syncytin b and its re... 17803826 - Does japanese encephalitis virus share the same cellular receptor with other mosquito-b... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of general virology Volume: 85 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2004 Mar |
Date Detail:
|
Created Date: 2004-03-02 Completed Date: 2004-04-30 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
|
Languages: eng Pagination: 701-7 Citation Subset: IM |
Affiliation:
|
Measles Virus Section, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS-C22, Atlanta, GA 30333, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line DNA, Viral / genetics Enzyme-Linked Immunosorbent Assay Genes, Reporter Genome, Viral* Humans Nipah Virus / genetics*, physiology RNA, Viral / genetics, isolation & purification Swine Swine Diseases / virology Transcription, Genetic Transfection Viral Proteins / genetics Virus Replication / genetics Zoonoses |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Viral; 0/RNA, Viral; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characteriza...
Next Document: Human respiratory syncytial virus matrix protein is an RNA-binding protein: binding properties, loca...