Document Detail


Nicotine and lipopolysaccharide affect cytokine expression from gingival fibroblasts.
MedLine Citation:
PMID:  17335378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: This in vitro study investigated the influence of nicotine, lipopolysaccharide (LPS), and a combination of both agents on cytokine expression from human gingival fibroblasts (HGFs). METHODS: HGFs were exposed for 48 hours to 250 microg/ml nicotine, 1 microg/ml Porphyromonas gingivalis LPS, or both. The expression of multiple cytokines was detected in the HGFs conditioned media using cytokine protein arrays. RESULTS: The untreated HGFs expressed several cytokines, which included relatively high levels of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1). They also expressed low levels of growth-regulated oncogene (GRO), IL-3, and IL-10. Nicotine had the greatest effect on the expression of GRO-alpha, IL-7, IL-10, and IL-15 compared to the untreated control. P. gingivalis LPS had the greatest effect on the expression of GRO-alpha; IL-7; IL-10; and RANTES (regulated on activation, normal T-cell expressed, and presumably secreted) compared to the untreated control. The combination of both agents had the biggest impact on the expression of GRO-alpha, IL-7, IL-10, IL-15, RANTES, and interferon-gamma (IFN-gamma) compared to the untreated control. CONCLUSION: HGFs exposed to nicotine, P. gingivalis LPS, or a combination of both agents increased the expression of multiple cytokines.
Authors:
Amjad Almasri; Kessiri Wisithphrom; L Jack Windsor; Byron Olson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of periodontology     Volume:  78     ISSN:  0022-3492     ISO Abbreviation:  J. Periodontol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-05     Completed Date:  2007-04-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  533-41     Citation Subset:  D; IM    
Affiliation:
Department of Periodontics, Indiana University School of Dentistry, Indianapolis, IN, USA.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Chemokine CCL2 / biosynthesis
Chemokine CCL5 / biosynthesis
Chemokine CXCL1
Chemokines, CXC / biosynthesis
Cytokines / biosynthesis*
Fibroblasts / drug effects,  metabolism
Gingiva / cytology,  drug effects*,  metabolism
Humans
Immunoenzyme Techniques
Interleukins / biosynthesis
Lipopolysaccharides / toxicity
Nicotine / toxicity*
Nicotinic Agonists / toxicity*
Porphyromonas gingivalis
Protein Array Analysis
Chemical
Reg. No./Substance:
0/CXCL1 protein, human; 0/Chemokine CCL2; 0/Chemokine CCL5; 0/Chemokine CXCL1; 0/Chemokines, CXC; 0/Cytokines; 0/Interleukins; 0/Lipopolysaccharides; 0/Nicotinic Agonists; 54-11-5/Nicotine

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