Document Detail


Nicotine and its metabolite cotinine are mitogenic for human vascular smooth muscle cells.
MedLine Citation:
PMID:  9129624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Intimal hyperplasia caused by smooth muscle cell (SMC) proliferation is the major cause of infrainguinal graft failure within the first 12 months. Tobacco smoking is associated with a twofold increase in graft failure within the first year of extremity bypass surgery, but the mechanism is not clearly understood. This study evaluated the effect of nicotine and its major stable metabolite cotinine on vascular SMC proliferation in vitro.
METHODS: SMC were harvested from human arteries and grown in culture with standard methods. Cells were seeded at a density of 1.8 x 10(4) cells/well in 24 multiwell dishes and cell cycle-synchronized. Subsequently the SMC were incubated with media containing 0.1% or 15% fetal bovine serum and nicotine or cotinine at concentrations ranging from 10(-9) mol/L to 10(-6) mol/L. Control samples were incubated with corresponding media but without the drugs. SMC proliferation was determined at 4 days with a cell counter. DNA synthesis was assessed at 24 hours with 3H-thymidine uptake. The results were expressed as a percentage change compared with the control samples (mean +/- SEM). Results were analyzed by analysis of variance and t tests.
RESULTS: In the presence of serum both nicotine and cotinine at concentrations of 10(-7) and 10(-8) mol/L were mitogenic for SMC in vitro (p < 0.05). A weak mitogenic effect was observed at a low serum concentration for cotinine but not nicotine. Cotinine at a concentration of 10(-9) mol/L, a level seen among passive smokers, was a statistically significant stimulus for DNA synthesis in both minimum serum and serum-supplemented media. At high concentrations both substances were toxic for the cells.
CONCLUSION: We have demonstrated a potential role for nicotine and cotinine in the development of intimal hyperplasia and ultimately failure of the vascular reconstruction.
Authors:
C S Carty; M Huribal; B U Marsan; J J Ricotta; M Dryjski
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of vascular surgery     Volume:  25     ISSN:  0741-5214     ISO Abbreviation:  J. Vasc. Surg.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-20     Completed Date:  1997-05-20     Revised Date:  2012-10-03    
Medline Journal Info:
Nlm Unique ID:  8407742     Medline TA:  J Vasc Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  682-8     Citation Subset:  IM    
Affiliation:
Department of Surgery, State University of New York at Buffalo, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Aorta / cytology
Cattle
Cell Count
Cell Division / drug effects
Cells, Cultured
Cotinine / adverse effects*
Culture Media
DNA / biosynthesis,  drug effects
Fetal Blood
Graft Survival
Humans
Hyperplasia
Iliac Artery / cytology
Mitogens / adverse effects*
Muscle, Smooth, Vascular / cytology,  drug effects*
Nicotine / adverse effects*
Smoking / adverse effects
Thymidine / metabolism
Tritium / diagnostic use
Tunica Intima / cytology,  drug effects
Vascular Surgical Procedures
Chemical
Reg. No./Substance:
0/Culture Media; 0/Mitogens; 10028-17-8/Tritium; 486-56-6/Cotinine; 50-89-5/Thymidine; 54-11-5/Nicotine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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