| Nicotine and its metabolite cotinine are mitogenic for human vascular smooth muscle cells. | |
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MedLine Citation:
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PMID: 9129624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Intimal hyperplasia caused by smooth muscle cell (SMC) proliferation is the major cause of infrainguinal graft failure within the first 12 months. Tobacco smoking is associated with a twofold increase in graft failure within the first year of extremity bypass surgery, but the mechanism is not clearly understood. This study evaluated the effect of nicotine and its major stable metabolite cotinine on vascular SMC proliferation in vitro. METHODS: SMC were harvested from human arteries and grown in culture with standard methods. Cells were seeded at a density of 1.8 x 10(4) cells/well in 24 multiwell dishes and cell cycle-synchronized. Subsequently the SMC were incubated with media containing 0.1% or 15% fetal bovine serum and nicotine or cotinine at concentrations ranging from 10(-9) mol/L to 10(-6) mol/L. Control samples were incubated with corresponding media but without the drugs. SMC proliferation was determined at 4 days with a cell counter. DNA synthesis was assessed at 24 hours with 3H-thymidine uptake. The results were expressed as a percentage change compared with the control samples (mean +/- SEM). Results were analyzed by analysis of variance and t tests. RESULTS: In the presence of serum both nicotine and cotinine at concentrations of 10(-7) and 10(-8) mol/L were mitogenic for SMC in vitro (p < 0.05). A weak mitogenic effect was observed at a low serum concentration for cotinine but not nicotine. Cotinine at a concentration of 10(-9) mol/L, a level seen among passive smokers, was a statistically significant stimulus for DNA synthesis in both minimum serum and serum-supplemented media. At high concentrations both substances were toxic for the cells. CONCLUSION: We have demonstrated a potential role for nicotine and cotinine in the development of intimal hyperplasia and ultimately failure of the vascular reconstruction. |
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Authors:
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C S Carty; M Huribal; B U Marsan; J J Ricotta; M Dryjski |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of vascular surgery Volume: 25 ISSN: 0741-5214 ISO Abbreviation: J. Vasc. Surg. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-05-20 Completed Date: 1997-05-20 Revised Date: 2012-10-03 |
Medline Journal Info:
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Nlm Unique ID: 8407742 Medline TA: J Vasc Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 682-8 Citation Subset: IM |
Affiliation:
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Department of Surgery, State University of New York at Buffalo, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Aorta / cytology Cattle Cell Count Cell Division / drug effects Cells, Cultured Cotinine / adverse effects* Culture Media DNA / biosynthesis, drug effects Fetal Blood Graft Survival Humans Hyperplasia Iliac Artery / cytology Mitogens / adverse effects* Muscle, Smooth, Vascular / cytology, drug effects* Nicotine / adverse effects* Smoking / adverse effects Thymidine / metabolism Tritium / diagnostic use Tunica Intima / cytology, drug effects Vascular Surgical Procedures |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Mitogens; 10028-17-8/Tritium; 486-56-6/Cotinine; 50-89-5/Thymidine; 54-11-5/Nicotine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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