Document Detail

Nicotine enlivenment of blood flow recovery following endothelial progenitor cell transplantation into ischemic hindlimb.
MedLine Citation:
PMID:  17784838     Owner:  NLM     Status:  MEDLINE    
Nicotine has been demonstrated to stimulate postnatal angiogenesis, having an antiapoptotic effect on endothelial cells. Given the extent of this angiogenesis-promoting effect, we hypothesized that nicotine may also stimulate postnatal vasculogenesis on endothelial progenitor cells (EPCs). Our analyses reveal some intriguing results using an in vitro assay with 2 x 10(-6) M of nicotine (smoker's average nicotine concentration and the dose of nicotine replacement therapy). The proliferation and migration activities of human EPCs cultured from peripheral blood mononuclear cells of non-smoking healthy volunteers were not affected by nicotine. The effect of nicotine on EPC survival was significantly enhanced under serum starvation on the ratio of Hoechest 33342-stained pyknotic nuclear cells as well as Annexin-V-stained cells to total cells. Furthermore, the antiapoptotic effect of nicotine was blocked completely by nicotinic acetylcholine receptor (nAChR) antagonist hexamethonium. Next, we verified how nicotine acts in vivo. Nicotine (100 ng/ml) was administered orally for 7 days before and 4 weeks after injection of cultured EPCs (1 x 10(5) /mouse) into the tail veins of 8-week-old athymic nude mice with ischemic hindlimbs. Laser doppler imaging analysis indicated that blood perfusion in the ischemic hindlimb was significantly enhanced in EPCs plus nicotine, as compared with EPCs alone. These findings suggest nicotine improves blood flow following EPC transplantation in patients with ischemic diseases.
Atsuhiko Sugimoto; Haruchika Masuda; Masamichi Eguchi; Hideki Iwaguro; Teruhisa Tanabe; Takayuki Asahara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells and development     Volume:  16     ISSN:  1547-3287     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-09-05     Completed Date:  2007-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  649-56     Citation Subset:  IM    
Department of Regenerative Medicine Science, Division of Cardiology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
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MeSH Terms
Apoptosis / drug effects*
Blood Flow Velocity / drug effects,  physiology*
Cell Culture Techniques
Cell Division / drug effects
Cell Movement / drug effects
Disease Models, Animal
Endothelial Cells / cytology,  drug effects,  transplantation*
Hindlimb / blood supply*
Ischemia / therapy*
Laser-Doppler Flowmetry
Leukocytes, Mononuclear / transplantation*
Nicotine / pharmacology*
Reg. No./Substance:

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