Document Detail


Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.
MedLine Citation:
PMID:  19469000     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.
METHODS: The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 microg/mL and 200 microg/mL) or 200 microg/mL nicotine plus 100 micromol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 (MMP-2) was analyzed by zymography and ELISA.
RESULTS: Nicotine (100 microg/mL, 200 microg/mL) enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine (200 microg/mL) stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2.
CONCLUSION: Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect of nicotine-enhanced esophageal squamous carcinoma cell migration and invasion.
Authors:
Ye Zong; Shu-Tian Zhang; Sheng-Tao Zhu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  15     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-26     Completed Date:  2009-07-13     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  2500-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Calcium Channel Blockers / pharmacology
Carcinoma, Squamous Cell* / metabolism,  pathology
Cell Line, Tumor / drug effects
Cell Movement / drug effects*
Cyclooxygenase 2 / genetics,  metabolism
Esophageal Neoplasms* / metabolism,  pathology
Humans
Matrix Metalloproteinase 2 / metabolism
Neoplasm Invasiveness / pathology*
Nicotine / pharmacology*
Nicotinic Agonists / pharmacology*
Smoking / adverse effects
Sulfonamides / pharmacology*
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Nicotinic Agonists; 0/Sulfonamides; 51803-78-2/nimesulide; 54-11-5/Nicotine; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 3.4.24.24/Matrix Metalloproteinase 2
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