Document Detail


Nicotine determination in mushrooms by LC-MS/MS with preliminary studies on the impact of drying on nicotine formation.
MedLine Citation:
PMID:  20234963     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A problem concerning significant amounts of nicotine in dried wild mushrooms (mainly Boletus edulis from China) has been reported to the European Commission. As a consequence, the European Food Safety Authority (EFSA) proposed temporary maximum residue levels (MRLs) of 0.036 mg kg(-1) for fresh wild mushrooms and 1.17 mg kg(-1) for dried wild mushrooms (2.3 mg kg(-1) for dried ceps only). The EFSA also highlighted the necessity for a monitoring and testing programme to be launched by food business operators at the start of the 2009 harvest season. In the present study, a quick and sensitive analytical method for routine analysis of nicotine in fresh and dried mushrooms was developed and validated by a single-laboratory procedure. The method, which employs an LC-MS/MS system and (+/-)-nicotine-d(4) as internal standard, has a limit of quantification of 6 and 60 microg kg(-1) for fresh and dried product, respectively. Analyses of samples spiked with different levels of nicotine showed recoveries ranging from 107 to 122%, with relative standard deviations of 2.9-10.1% depending on the spiking level. The combined uncertainties, calculated at a low level for frozen (0.015 mg kg(-1)) and a high level for the dried (2 mg kg(-1)) matrix, were 13 and 10%, respectively. Application of the method to real samples of mushrooms purchased on the market or obtained from local producers showed nicotine levels ranging 0.01-0.04 and 0.1-4.5 mg kg(-1) in fresh/frozen and dried matrices, respectively. To establish reasons for the unexpectedly high levels of the nicotine in dried matrices, preliminary laboratory experiments involving drying mushrooms were performed under various conditions.
Authors:
Chiara Cavalieri; Luciana Bolzoni; Mirella Bandini
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment     Volume:  27     ISSN:  1944-0057     ISO Abbreviation:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485040     Medline TA:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Country:  England    
Other Details:
Languages:  eng     Pagination:  473-7     Citation Subset:  IM    
Affiliation:
Stazione Sperimentale per l'Industria delle Conserve Alimentari (SSICA), Parma, Italy. chiara.cavalieri@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Agaricales / chemistry*
Chromatography, High Pressure Liquid / methods*
Food Contamination / analysis*
Food, Preserved / analysis*
Frozen Foods / analysis
Maximum Allowable Concentration
Nicotine / analysis*
Reproducibility of Results
Tandem Mass Spectrometry / methods*
Chemical
Reg. No./Substance:
54-11-5/Nicotine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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