| Nicotine combined with okadaic acid or taxol adversely affects bovine oocyte maturation and subsequent embryo development. | |
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MedLine Citation:
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PMID: 18793772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the effects of nicotine in combination with okadaic acid (OA) or taxol on bovine oocyte maturation and subsequent embryonic development. DESIGN: Prospective randomized study. SETTING: University research laboratory. PATIENT(S): Bovine ovaries, oocytes, and embryos. INTERVENTION(S): Oocyte maturation and subsequent embryo development in the presence of nicotine in combination with okadaic acid and taxol. MAIN OUTCOME MEASURE(S): Haploid composition, cleavage rate, IVF and parthenogenetic embryo development, blastocyst cell number. RESULT(S): The results showed that nicotine and OA or nicotine and taxol significantly decreased oocyte maturation rates. Combinations of nicotine (10 or 50 micromol/L) and OA (0.01 or 0.05 micromol/L) did not affect oocyte haploid composition; however, taxol alone or combined with nicotine caused a decrease in haploid composition compared with control. Parthenogenetic activation of oocytes that were matured in nicotine, OA, or taxol resulted in blastocyst development rates of 12%-17%, which were not different from the control. Various combinations of nicotine and OA or nicotine and taxol significantly lowered (3%-8%) blastocyst development compared with control. The average cell number of blastocysts derived from nicotine + OA- and nicotine + taxol-treated oocytes was lower than all other treatment groups and control. For oocytes fertilized in vitro, oocytes matured in OA, taxol, or combinations of nicotine and OA or taxol-containing media resulted in significantly lower cleavage rates and blastocyst development compared with the control group and the 10 micromol/L nicotine treatment group. The IVF embryos cultured in nicotine + OA-containing medium had a significantly lower blastocyst development rate. CONCLUSION(S): Combinations of nicotine with OA or taxol adversely affect oocyte maturation and subsequently result in poor blastocyst development. |
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Authors:
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Guang-Peng Li; Shufen Yang; Ying Liu; Benjamin R Sessions; Kenneth L White; Thomas D Bunch |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-14 |
Journal Detail:
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Title: Fertility and sterility Volume: 92 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-03 Completed Date: 2009-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 798-805 Citation Subset: IM |
Affiliation:
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Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, People's Republic of China. guangpengli@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cells, Cultured Dose-Response Relationship, Drug Drug Synergism Embryonic Development / drug effects*, physiology* Female Nicotine / toxicity* Okadaic Acid / toxicity* Oocytes / drug effects*, growth & development* Paclitaxel / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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33069-62-4/Paclitaxel; 54-11-5/Nicotine; 78111-17-8/Okadaic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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