Document Detail


Nicotine- and Tar-free Cigarette Smoke Extract Induces Cell Injury via Intracellular Ca(2+)-Dependent Subtype-Specific Protein Kinase C Activation.
MedLine Citation:
PMID:  23149578     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Nicotine- and tar-free cigarette smoke extract (CSE) is reported to induce cell damage via activation of protein kinase C (PKC) and NADPH oxidase (NOX) in rat C6 glioma cells. Here we determined PKC isozyme(s) activated by CSE and their activation mechanism. In C6 glioma cells, mRNAs for PKCα, PKCδ, PKCε, and PKCι were expressed. CSE triggered translocation of PKCα and PKCε to plasma membrane. CSE-induced cell damage and PKC translocation were inhibited by chelating intracellular Ca(2+) but not extracellular Ca(2+). These results suggest that CSE induces cell damage through intracellular Ca(2+)-dependent activation of PKCα and PKCε and subsequent NOX activation.
Authors:
Yosuke Mai; Tsunehito Higashi; Koji Terada; Chizuru Hatate; Prabha Nepal; Mika Horiguchi; Takuya Harada; Soichi Miwa; Takahiro Horinouchi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-13
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  -     ISSN:  1347-8648     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan.
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