Document Detail

Nicotinamide mononucleotide adenylyltransferase activity in human erythrocytes.
MedLine Citation:
PMID:  8470897     Owner:  NLM     Status:  MEDLINE    
Nicotinamide mononucleotide adenylyltransferase (NMN-AT) activity has not hitherto been demonstrated in human red blood cells, owing to its low activity. Since it is usually located in the nucleus, the possibility of finding it in human erythrocytes was excluded. Here we report the first demonstration and characterization of NMN-AT in human red blood cells, by an HPLC method. The enzyme is Mg2+ dependent, with a Km of 0.303 mM for nicotinamide mononucleotide and 0.103 mM for ATP, and a Vmax of 346 nmol g Hb-1 h-1. The crude preparation is also active on nicotinic acid mononucleotide, producing nicotinic acid adenine dinucleotide. NMN-AT activity is inhibited by nicotinic acid mononucleotide, and nicotinic acid adenylyltransferase is inhibited by nicotinamide mononucleotide. Fiftyfold purification of NMN-AT was achieved by DEAE-Toyopearl chromatography, and the kinetic characteristics were determined. The partially purified preparation maintained its nicotinic acid adenylyltransferase activity. These findings are discussed in light of the regulation of NAD metabolism in human red blood cells.
S Sestini; C Ricci; V Micheli; G Pompucci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  302     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-05-12     Completed Date:  1993-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  206-11     Citation Subset:  IM    
Dipartimento di Biologia Molecolare, Università di Siena, Italy.
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MeSH Terms
Adenosine Triphosphate / metabolism
Anion Exchange Resins
Chromatography, High Pressure Liquid
Enzyme Stability
Erythrocytes / enzymology*
Magnesium / pharmacology
Nicotinamide Mononucleotide / analogs & derivatives,  metabolism,  pharmacology
Nicotinamide-Nucleotide Adenylyltransferase / antagonists & inhibitors,  blood*
Resins, Synthetic
Substrate Specificity
Reg. No./Substance:
0/Anion Exchange Resins; 0/Resins, Synthetic; 1094-61-7/Nicotinamide Mononucleotide; 122985-81-3/Toyopearl DEAE 650 M; 321-02-8/nicotinate mononucleotide; 56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium; EC Adenylyltransferase

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