Document Detail


Nicotiana attenuata SIPK, WIPK, NPR1, and fatty acid-amino acid conjugates participate in the induction of jasmonic acid biosynthesis by affecting early enzymatic steps in the pathway.
MedLine Citation:
PMID:  19897603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wounding and herbivore attack elicit the rapid (within minutes) accumulation of jasmonic acid (JA) that results from the activation of previously synthesized biosynthetic enzymes. Recently, several regulatory factors that affect JA production have been identified; however, how these regulators affect JA biosynthesis remains at present unknown. Here we demonstrate that Nicotiana attenuata salicylate-induced protein kinase (SIPK), wound-induced protein kinase (WIPK), nonexpressor of PR-1 (NPR1), and the insect elicitor N-linolenoyl-glutamate [corrected] (18:3-Glu) participate in mechanisms affecting early enzymatic steps of the JA biosynthesis pathway. Plants silenced in the expression of SIPK and NPR1 were affected in the initial accumulation of 13-hydroperoxy-linolenic acid (13-OOH-18:3) after wounding and 18:3-Glu elicitation by mechanisms independent of changes in 13-lipoxygenase activity. Moreover, 18:3-Glu elicited an enhanced and rapid accumulation of 13-OOH-18:3 that depended partially on SIPK and NPR1 but was independent of increased 13-lipoxygenase activity. Together, the results suggested that substrate supply for JA production was altered by 18:3-Glu elicitation and SIPK- and NPR1-mediated mechanisms. Consistent with a regulation at the level of substrate supply, we demonstrated by virus-induced gene silencing that a wound-repressed plastidial glycerolipase (NaGLA1) plays an essential role in the induction of de novo JA biosynthesis. In contrast to SIPK and NPR1, mechanisms mediated by WIPK did not affect the production of 13-OOH-18:3 but were critical to control the conversion of this precursor into 12-oxo-phytodienoic acid. These differences could be partially accounted for by reduced allene oxide synthase activity in WIPK-silenced plants.
Authors:
Mario Kallenbach; Fiammetta Alagna; Ian Thomas Baldwin; Gustavo Bonaventure
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-06
Journal Detail:
Title:  Plant physiology     Volume:  152     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-30     Completed Date:  2010-03-30     Revised Date:  2010-09-28    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  96-106     Citation Subset:  IM    
Affiliation:
Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Jena 07745, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry,  metabolism*
Cyclopentanes / metabolism*
Fatty Acids / chemistry,  genetics,  metabolism*
Gene Expression Regulation, Plant / physiology
Gene Silencing
Lipase / genetics,  metabolism
Oxylipins / metabolism*
Plant Leaves / genetics,  metabolism
Plant Proteins / genetics,  metabolism*
Tobacco / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cyclopentanes; 0/Fatty Acids; 0/Oxylipins; 0/Plant Proteins; 6894-38-8/jasmonic acid; EC 3.1.1.3/Lipase
Comments/Corrections
Comment In:
Plant Signal Behav. 2010 Mar;5(3):287-9   [PMID:  20037473 ]
Erratum In:
Plant Physiol. 2010 Mar;152(3):1760

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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