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Nicotiana attenuata α-DIOXYGENASE1 through its production of 2-hydroxylinolenic acid is required for intact plant defense expression against attack from Manduca sexta larvae.
MedLine Citation:
PMID:  22937952     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Nicotiana attenuata α-DIOXYGENASE1 (α-DOX1) is an oxylipin-forming gene elicited during herbivory by fatty acid amino acid conjugates (FACs) contained in oral secretions of Manduca sexta. To understand the roles of Naα-DOX1 and its major product, 2-hydroxylinolenic acid (2-hydroxylinolenic acid), in N. attenuata's anti-herbivore defenses, we used a transgenic line specifically silenced in Naα-DOX1 expression (ir-α-dox1) and monitored 2-HOT production in M. sexta-damaged tissues and its role in influencing the production of direct defense compounds and resistance to this insect. Attack by M. sexta larvae amplified 2-HOT formation at the feeding sites; a reaction probably facilitated by Naα-DOX1's high pH optimum which allows 2-HOT formation to occur in the more alkaline conditions at the feeding sites or potentially in the insect mouth parts after the leaf tissue is ingested. Manduca sexta larvae performed better on ir-α-dox1 plants than on wild-type (WT) plants as a result of attenuated herbivory-specific JA and 2-HOT bursts as well as JA-inducible well-established defenses (nicotine, caffeoylputrescine and trypsin proteinase inhibitors). Repeated applications of 2-HOT to wounds before insect feeding partly amplified JA-controlled defenses and restored the resistance of ir-α-dox1 plants. We conclude that 2-HOT, produced by attack-activated α-DOX1 activity, participates in defense activation during insect feeding.
Authors:
Emmanuel Gaquerel; Anke Steppuhn; Ian T Baldwin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-31
Journal Detail:
Title:  The New phytologist     Volume:  -     ISSN:  1469-8137     ISO Abbreviation:  New Phytol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-9-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882884     Medline TA:  New Phytol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors New Phytologist © 2012 New Phytologist Trust.
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Strasse 8, D-07745, Jena, Germany.
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